Table 1.

Interaction of nuclear export receptors with nucleoporin CAN in yeast cellsa

DNA binding domain fusionInduced β-Gal activityb withc:
AD-CAN (1864–2090)AD-CAN (1805–2090)AD-CAN (1600–2090)AD-importin α2
GAL4<10<10<10<10
GAL4-Crm19,93628,0649,880<10
GAL4-Tap(61–619)32902232<10
GAL4-CAS<10<10<108,234
  • a The ability of the indicated GAL4 DNA binding domain fusions and GAL4-AD fusions to interact in vivo was measured using the yeast two-hybrid assay as described for Fig. 2A.

  • b In milli-optical density units per milliliter of lysate.

  • c The residues from each protein used in the relevant fusions are indicated. If no residue numbers are shown, then the full-length protein was analyzed.