Table 1.

Cytofluorimetric analysis of murine leukocyte populations 6 days following infection with HK/483, HK/486, or PR/8

TissueVirusaTotal no. of cellb/tissueMean % of cells ± SEc
CD4+CD8+CD3+CD45R/B220+
LungPR/81.1 × 107 32.4 ± 0.913.0 ± 0.641.2 ± 3.940.1 ± 1.9
HK/4861.2 × 107 25.8 ± 1.610.5 ± 0.439.4 ± 2.644.0 ± 2.4
HK/4835.3 × 106d 19.0 ± 0.6e 6.6 ± 0.6e 30.9 ± 1.7e 34.0 ± 3.8
SpleenNone4.6 × 107 25.3 ± 1.612.6 ± 0.435.9 ± 1.353.0 ± 0.6
PR/84.8 × 107 25.7 ± 2.114.8 ± 0.342.5 ± 2.753.1 ± 3.4
HK/4864.9 × 107 25.4 ± 2.413.3 ± 1.235.8 ± 5.157.4 ± 4.7
HK/4833.4 × 107e 22.6 ± 2.210.2 ± 1.734.6 ± 2.660.8 ± 2.7
MLNNone8.1 × 105 35.5 ± 0.510.8 ± 0.651.3 ± 0.647.1 ± 0.3
HK/4862.1 × 106 38.2 ± 0.914.2 ± 1.045.8 ± 0.752.4 ± 0.1
HK/4831.1 × 106 28.4 ± 2.7e 8.9 ± 1.4e 35.1 ± 3.5e 68.4 ± 0.7
ThymusNone4.5 × 107 12.8 ± 1.04.9 ± 0.9 (81.7)20.6 ± 1.6ND
HK/4865.4 × 107 13.2 ± 2.56.9 ± 1.2 (77.6)19.8 ± 1.1ND
HK/4831.4 × 107d 37.0 ± 4.7d 17.2 ± 2.7e (14.1)d 64.4 ± 2.4d ND
  • a BALB/c mice were infected i.n. with 100 MID50 of the indicated viruses.

  • b Mice from each group were euthanized on day 6 p.i., and individual tissues were treated with collagenase. The total cell numbers in each of the tissues were determined by staining with Turks solution and microscopic examination.

  • c Single-cell suspensions were analyzed by flow cytometry for percent of CD3+, CD4+, or CD8+ T cells and CD45R (B220)+ B cells. Values are representative of three independent experiments and are the mean ± SE of two to three mice from each group. Values in parentheses represent the percentages of CD4-CD8 double-positive thymocytes.

  • d P < 0.005 compared with HK/486-infected group.

  • e P < 0.05 compared with HK/486-infected group.