Table 1.

Analysis of partially purified virusa

Virusbp24 Ag (ng/ml)cRT activity (nU/μl)dp24 Ag/RTeVirus replicationf (%)
TAR11153.038.3Negative
TAR2922.143.8Negative
TAR31032.442.9Negative
TAR42577.832.93
TAR51,21226.945.05
TAR62618.231.7100
Wild type71415.845.2100
  • a The results are representative of those from three independent experiments.

  • b The TAR RNA sequence is shown in Fig. 1.

  • c Concentration of p24 Ag measured in virus pelleted through a 20% sucrose cushion.

  • d RT activity of virus pelleted through a 20% sucrose cushion.

  • e Absolute value of p24 Ag concentration divided by RT activity.

  • f Peak p24 Ag concentration in culture supernatant relative to the wild-type level.