Table 1.

Proteolytic cleavage and intermolecular disulfide bond formation in full-length and loop-deleted WT and SOS gp140 proteinsa

gp140 mutantgp140 cleavageDisulfide bond formation
WT proteinSOS protein
WT, full length+++++++
ΔV1++++++
ΔV2+++++++
ΔV3++++
ΔV1V2′+++++++
ΔV1V2* +++++
ΔV1V2′V3+++
ΔV1V2*V3++++++
  • a The original data are presented in Fig.3 and 4. The extent of gp140 cleavage in the presence of cotransfected furin was determined. +++, 90 to 100% cleavage; ++, 60 to 90% cleavage; +, 30 to 60% cleavage; −, 0 to 30% cleavage. The formation of intermolecular disulfide bonds was determined in SOS gp140.