T-cell proliferation after adoptive reconstitution of LCMV carrier mice with spleen cells from LCMV-immune or naivegld mice
| Expt | Reconstitutiona | Proliferationb |
|---|---|---|
| 1 | LCMV-naivegld | 222 ± 11 |
| LCMV-immune gld | 53 ± 4.4 | |
| 2 | None | 212 ± 17 |
| LCMV-immune C57BL/6 | 2.5 ± 15 | |
| LCMV-immunegld | 141 ± 3.3 | |
| 3 | None | 174 ± 36 |
| LCMV-immune C57BL/6 | 2.2 ± 0.1 | |
| LCMV-immunegld | 179 ± 12 | |
| 4 | LCMV-immune C57BL/6 | 5.9 ± 2.0 |
| LCMV-immunegld | 41 ± 5.1 |
a LCMV carrier mice were reconstituted with 3 × 107 to 4 × 107 of the indicated C57BL/6 or congenic gld mutant spleen cells.
b Numbers represent 103 mean cpm ± standard deviation after a 6-h pulse with 1 μCi of [3H]thymidine after 2 days of culture with plate-bound anti-CD3 antibody.