TABLE 1

Purified N9 MAbs for in vitro and in vivo studies

MAbIC50 (ng/ml)aMutation in NA of selected mutantsKD (nM)b
ELLAMU-NANA
1E8c>4,000>32,000K434E or K434R>335d
2E635.5>32,000P251H2.4
2F620.6>32,000D253N3.7
5C926.5>32,000G250E or P251H93.2
5H11142.9>32,000N345I or K434E>335
7A4161.2>32,000D253Y16.8
7F8>4,000>32,000N334S46.9
7F12305.5>32,000S371L or K434E>335
10F447.6643K434E1.4
11B2>4,000>32,000S247P250
  • a ELLAs and MU-NANA assays were performed with reassortant H6N9AH/13, which bears the HA gene of the H6N2 virus A/turkey/Massachusetts/3740/1965, the NA of AH/13, and the internal genes of the A(H1N1) virus A/Puerto Rico/8/1934 (PR8).

  • b KD values were measured with SPR using recombinant AH/13 NA.

  • c MAbs 1E8, 2F6, 10F4, and 11B2 were selected for in vivo experiments.

  • d No binding was detected by SPR at the highest MAb concentration tested, 50 μg/ml (335 nM).