Table 1.

Flow cytometry analysis of surface expression of chimeric proteins

Sample no.Construct% CY5 positiveCY5 MCFGFP MCFMCF ratioa
1 VSVGb 0.01.97224.450.01
2 VSVGc 0.02.91231.460.01
3 B5R0.13.21140.100.02
4 VSVG79.6170.84218.940.78
5 G/B/B14.934.49359.100.10
6 G/B/G38.389.83234.710.38
7 G/G/B5.716.29389.950.04
8 G/G/Δ7.717.26293.560.06
9 G/B/Δ40.3104.99399.240.26
10G/B/BY310A 47.2109.84533.140.21
11G/B/BΔ315/316 29.860.21328.190.18
12G/B/BY310A/Δ315/316 43.4100.48473.170.21
  • a CY5 MCF/GFP MCF.

  • b Neither MAb I1 nor CY5-conjugated secondary antibody was added to the cells.

  • c Only CY5-conjugated secondary antibody was added to the cells.