Table 4.

T-cell proliferation after adoptive reconstitution of LCMV carrier mice lacking the IFN-γ receptor

ExptReconstitutionaProliferationb
1None196 ± 18
LCMV naive116 ± 13
LCMV immune36 ± 1.8
Controlc 2.2 ± 0.1
2NoneNDd
LCMV naive115 ± 0.8
LCMV immune47 ± 21
Controlc 4.7 ± 0.9
3None154 ± 17
LCMV naive100 ± 15
LCMV immune46 ± 8.9
Controlc 11 ± 2.2
  • a IFN-γ receptor knockout LCMV carrier 129/SV mice were reconstituted with 3 × 107 of the indicated C57BL/6 spleen cells.

  • b Numbers represent 103 mean cpm ± standard deviation after a 6-h pulse with 1 μCi of [3H]thymidine after 2 days of culture with plate-bound anti-CD3.

  • c Wild-type LCMV carrier C57BL/6 mice reconstituted with 3 × 107 of the same LCMV-immune spleen cells used to reconstitute persistently infected IFN-γ receptor knockout mice. Wild-type LCMV carrier normal 129/SV controls were not available for this experiment, but we have documented the high sensitivity of T cells from acutely LCMV-infected 129/SV mice elsewhere (19).

  • d ND, not done.