Table 1.

PCR primers used in this study

Gene (assay)PrimersaCycles [no. (°C/s)]MgCl2 (mM)Product size (bp)
IFN-β (genotyping)5′-TGGGAAATTCCTCTGAGGCAG-3′ (S)35 (94/30, 60/30, 72/60)1.5–3717
5′-CACTCATTCTGAGGCATCAACTGAC-3′ (A)
GFP (genotyping)5′-GGTGAAGGTGATGCAACATACGG-3′ (S)35 (94/30, 60/30, 72/60)1.5–3513
5′-TGTGGACAGGTAATGGTTGTCTGG-3′ (A)
IFN-β (RT-PCR)5′-ACACAAGCTTAACCACCATGAACAACAGGTGGATCCTCCACGC-3′ (S)30 (94/30, 60/30, 72/60)3560
5′-GTTAGGAATTCTCAGTTTTGGAAGTTTCTGGTAAGTCTTCG-3′ (A)
GFP (RT-PCR)5′-GGTGAAGGTGATGCAACATACGG-3′ (S)30 (94/30, 60/30, 72/60)3513
5′-TGTGGACAGGTAATGGTTGTCTGG-3′ (A)
Universal IFN-α5′-ATGGCTAGGCYCTGTGCTTTC-3′ (S)30 (94/60, 50/120, 72/180)1.5∼500
5′-TCTGAYCACCTCCCAGGCACA-3′ (A)
IFN-non-α45′-ARSYTGTSTGATGCARCAGGT-3′ (S)30 (94/30, 55/30, 72/30)1.5∼104
5′-GGWACACAGTGATCCTGTGG-3′ (A)
IFN-α45′-CTGGTCAGCCTGTTCTCTAGGATG-3′ (S)30 (94/30, 55/30, 72/30)1.5314
5′-TCAGAGGAGGTTCCTGCATCAC-3′ (A)
2-5A synthetase5′-CCCCATCTGCATCAGGAGGTGGAG-3′ (S)30 (94/30, 58/60, 72/60)1.5422
5′-AAGTCATAATACTTTGTCCAGTAG-3′ (A)
PGK5′-CCTCCGCTTTCATGTAGAGGAAGA-3′ (S)30 (94/60, 55/60, 72/60)1.5400
5′-GTAAAGGCCATTCCACCACCAA-3′ (A)
  • a S, sense; A, antisense.