Table 1.

PBL of MVA-gag-pol-vaccinated Mamu-A*01+ rhesus macaques demonstrate tetramer+ CD8+ T cells and functional CTL

ImmunizationMonkeyAssayDay postimmunization
3rd4th
071728071428
MVA-Gag-Pol442Fresha 0.1 NTd 0.60.80.20.50.50.3
Peptide stimulatedb 20.8NT58.350.439.941.245.946.9
% Specific lysisc 48NT615865697459
443Fresh0.82.41.72.00.80.90.80.5
Peptide stimulated4.823613968586977
% Specific lysis1944575667725467
446Fresh0.11.30.30.20.20.40.40.4
Peptide stimulated4357413744515359
% Specific lysis5558566062755357
449Fresh0.11.61.00.60.20.91.30.8
Peptide stimulated1316252324395952
% Specific lysis3637573248685253
Control MVA454Fresh0.00.00.00.00.00.00.00.0
Peptide stimulated0.10.10.20.50.50.50.30.2
% Specific lysisNT0200000
538Fresh0.00.00.00.0
Peptide stimulated0.81.11.20.5
% Specific lysis0000
564Fresh0.0NTNT0.0
Peptide stimulated1.2NTNT0.3
% Specific lysis1NTNT0
565Fresh0.0NTNT0.0
Peptide stimulated1.1NTNT0.4
% Specific lysis1NTNT0
567Fresh0.0NTNT0.0
Peptide stimulated1.2NTNT0.3
% Specific lysis2NTNT0
  • a Lymphocytes were assessed for binding of tetramer to CD8αβ+ T cells in whole blood.

  • b PBL were cultured with p11C prior to assessment of tetramer binding to CD8αβ+ T cells at day 7.

  • c PBL were cultured with p11C, and 7 days later p11C, C-M-specific lysis was determined in a standard 51Cr release assay. Data represent percent p11C, C-M-specific lysis (background subtracted at an effector/target ratio of 2.5:1.

  • d NT, not tested.