Table 1.

Primers and probes used in RT-PCR analyses

Transcript (protein)Primers and probeGenome coordinatesSequence
BKRF1 (EBNA-1)5′ Primer (U exon)67483–675025′-TTAGGAAGCGTTTCTTTGAGC-3′
3′ Primer (K exon)107986–1079675′-CATTTCCAGGTCCTGTACCT-3′
Probe (U exon)67544–675635′-AGAGAGTAGTCTCAGGGCAT-3′
BZLF-1 (ZEBRA)5′ Primer (exon 1)102719–1027005′-TTCCACAGCCTGCACCAGTG-3′
3′ Primer (exon 2/3 splice)102330–102341 and 102426–1024335′-GGCAGCAGCCACCTCACGGT-3′
Probe (exon 2)102450–1024695′-CTTAAACTTGGCCCGGCATT-3′
BHRF-1 (EA)5′ Primer (exon 1)54461–544805′-TTCTCTTGCTGCTAGCTCCA-3′
3′ Primer (exon 1)53830–538495′-GTCAAGGTTTCGTCTGTGTG-3′
Probe (exon 1)54411–544355′-ATGCACACGACTGTCCCGTATACAC-3′
BALF-2 (EA)5′ Primer (exon 1)163094–1630725′-GTCAAGATGTTCAAGGACGTGG-3′
3′ Primer (exon 1)162856–1628785′-CTCATAGCACATACAGATGGGC-3′
Probe (exon 1)162911–1629305′-GCGGTAAAACAGCTGGGTGA-3′
BcLF-1 (VCA)5′ Primer (exon 1)136231–1362105′-TATGCCCAATCCCAAGTACACG-3′
3′ Primer (exon 1)135867–1358885′-TGGACGGGTGGAGGAAGTCTTC-3′
Probe (exon 1)136119–1361385′-ACGCGAGGAGGAGGTTATTC-3′
GAPDH5′ Primer (exon 8)3070–30895′-ACCACAGTCCATGCCATCAC-3′
3′ Primer (exon 9)3605–35275′-TCCACCACCCTGTTGCTGTA-3′
Probe (exon 8)3218–32455′-CACGGAAGGCCATGCCAGTGAGCTTCCCGT-3′