Table 1.

Colony reduction assaya

Cell lineHPV DNA statusbStatus of gene forc:No. of puromycin-resistant colonies after transfection with:
pRbp53VectorE2TA plasmidE2TR plasmid
HeLa+wtwt74070762
SiHa+wtwt11110117
C33Amutmut1,4241,4861,392
U20Swtwt564552452
  • a The indicated cell lines were transfected with vector alone or E2TA or E2TR plasmids, along with pBabe-puro. Twenty-four hours after transfection, cell cultures were divided between two 10-cm-diameter plates, allowed to attach, and placed under selection in media containing 0.7 μg of the selection antibiotic/ml. The number of drug-resistant colonies was determined after 3 weeks.

  • b +, positive; −, negative.

  • c wt, wild type; mut, mutant.