Table 1.

Production of virus by full-length BVDV genomic RNA transcripts containing either wild type or mutations in NS3a

ConstructVirus titer (PFU/ml)
pACCX-BVDV1.8 × 106
pVVNADL1.8 × 106
pCLK (GKT→GAT)0
pCLM21 (DEYH→DEYA)0
pCLΔ21 (delete motif VI)0
  • a Yields of virus are from supernatants derived from cells 4 days posttransfection with genomic RNA transcripts from either wild type (pACCX-BVDV or pVVNADL) or NS3 mutants (pCLK, pCLΔ21, or pCLM21).