Table 1.

Extracellular virus production from neurons versus that from nonneuronal cellsa

Type of celld.p.i.% Infected cellsbPFU/mlcPFU/1,000 infected cellsd
Primary CD46+ mouse neuron15NDe 0
252010
310206
Vero180ND0
21006.0 × 104 600
31003.2 × 105 1,600
HeLa15036010
2807.2 × 103 130
31001.6 × 104 170
NT2150542
285361
3901563
Undifferentiated NT2160401
2756.4 × 103 130
3959.2 × 103 100
  • a Cells plated at a density of 1 × 105 to 2 × 105 cells/coverslip were infected 1 day later with MV Edmonston (MOI = 1). Data represent results from at least two separate experiments, each performed in duplicate.

  • b Percent infected cells was determined by immunohistochemical staining of MV antigen.

  • c Supernatants tested for infectious virus by plaque assay.

  • d PFU/1,000 cell values, used to correct for cell division rates in fibroblast cultures (cell counts determined in parallel uninfected cultures) and the proportion of cells infected at the indicated time, were calculated as {[(PFU/ml) × 4 ml]/(number of cells × proportion infected)} × 1,000.

  • e ND, not detected.