Table 2.

Recombinant viruses

VirusPlaque diam (mm) with:Heparin-Sepharose elution (mM NaCl)ELISA reactivityb of E2 MAb:
AgarAgaroseR6G5202
Toto 11011.3 ± 0.4a 3.3 ± 0.7323+++
R157H2.5 ± 0.72.8 ± 0.6289+++
N62D2.0 ± 0.72.2 ± 0.6276++
K159E2.8 ± 0.82.6 ± 0.7261++
K230M2.4 ± 0.82.6 ± 0.7202+++
K76N2.3 ± 0.92.5 ± 0.7142+++
K76T2.3 ± 0.82.6 ± 0.897+++
K76E3.1 ± 0.82.5 ± 0.866+++
  • a The plaque size (mean ± standard deviation) of Toto 1101 under agar was significantly smaller (P, <0.05) than those of all other viruses under agar and was also significantly smaller than that of Toto 1101 under agarose (Kruskal-Wallis analysis of variance ranks followed by Dunn's multiple-comparison test).

  • b +, reactivity; −, no reactivity.