Table 2.

Summary of results of three- and four-color immunophenotyping of CD4+ T cells in normal macaque tissues

GateNo. of animals examinedLymphocyte subset% T cell expressing marker (mean ± SD)
LPLIELLymph nodesSpleenBlood
JejunumIleumColonJejunumIleumColonAxilliaryMesenteric
CD3+CD4+ 6CD45RA+ 4.9 ± 2.113.0 ± 1.914.2 ± 7.37.5 ± 5.520.4 ± 16.527.6 ± 17.852.0 ± 13.455.3 ± 12.447.6 ± 9.961.3 ± 3.1
CD3+CD4+ 6L-selectin+ 5.5 ± 4.36.2 ± 2.09.2 ± 3.99.2 ± 4.314.4 ± 10.618.0 ± 13.038.6 ± 15.228.0 ± 7.420.8 ± 6.862.7 ± 8.1
CD3+ CD4+ 6DP 45RA+ L-selectin+ 2.2 ± 1.33.4 ± 0.74.1 ± 1.04.1 ± 3.69.6 ± 11.012.6 ± 11.228.6 ± 14.520.8 ± 7.316.2 ± 6.550.7 ± 6.1
CD4+ 4CD69+ 93.0 ± 4.879.3 ± 6.177.5 ± 3.797.5 ± 1.793.8 ± 3.374.0 ± 25.927.2 ± 7.736.0 ± 11.524.0 ± 0.03.2 ± 2.7
CD4+ 8CD25+ 24.9 ± 12.414.3 ± 8.917.0 ± 7.718.4 ± 10.719.8 ± 16.916.8 ± 11.28.4 ± 3.310.3 ± 3.48.0 ± 4.75.8 ± 3.8
CD3+CD4+ 8HLA-DR+ 48.3 ± 36.542.9 ± 32.633.7 ± 29.135.8 ± 34.036.8 ± 27.433.7 ± 29.126.3 ± 18.521.0 ± 15.036.6 ± 24.719.4 ± 13.6
CD3+CD4+ 8CD38+ 82.0 ± 12.884.9 ± 6.877.9 ± 10.183.3 ± 10.182.3 ± 9.883.8 ± 7.385.7 ± 6.296.2 ± 1.580.2 ± 7.485.8 ± 10.0
CD4+ 8CD28+ 82.3 ± 15.688.6 ± 13.595.0 ± 4.252.1 ± 24.169.8 ± 27.976.2 ± 25.996.6 ± 2.995.4 ± 4.589.2 ± 5.191.6 ± 2.4