TABLE 2

Overview of PB1 mutant characteristics relative to the wild typea

EnzymeRNP reconstitutionExtension on vRNAExtension on cRNARNA cleavageTrimer formation
vRNAmRNAFLFR
Wild type+++++++++++++++++++++
S269A+++++++++++NT+++
L271ANTNTNT++++++NT+++
P272ANTNTNTNT+++
V273A+++++++++++NT+++
Δ648–651++++++++++++++++++
Δ631–662+
Δ642–656+++++++++++++++
Δ656–662+++
Δ631–642++++++++
Δ636–642+++++++++++++++
Δ631–635++++++
  • a ++++, >100% of the value for the wild type; +++, 75 to 100% of the value for the wild type; ++, 50 to 75% of the value for the wild type; +, 25 to 50% of the value for the wild type; −, 0 to 25% of the value for the wild type; NT, not tested.