TABLE 1

Primers used for PB1 mutagenesis and primer extension

MutantPrimer directionaSequence (5′–3′)
PB1 Δ648–651FwGAACAATGCAGTGATAATGCCAGGCGCCAAAAACATGGAGTATGATGC
RvGCATCATACTCCATGTTTTTGGCGCCTGGCATTATCACTGCATTGTTC
PB1 Δ631–662FwGCAACCCACTGAACCCAACACACTCCTGGATCCCC
RvGGGGATCCAGGAGTGTGTTGGGTTCAGTGGGTTGC
PB1 Δ642–656FwAACCATAAAGACATTGAATCAGTGAACTATGATGCTGTTGCAACAACA
RvTGTTGTTGCAACAGCATCATAGTTCACTGATTCAATGTCTTTATGGTT
PB1 Δ656–662FwATGGTCCAGCCAAAAACATGACACACTCCTGGATCCCC
RvGGGGATCCAGGAGTGTGTCATGTTTTTGGCTGGACCAT
PB1 Δ631–642FwGCAACCCACTGAACCCAGCAGTGATAATGCCAGCAC
RvGTGCTGGCATTATCACTGCTGGGTTCAGTGGGTTGC
PB1 Δ636–642FwCTGAACCCATTTGTCAACCATAAAGCAGTGATAATGCCAGCAC
RvGTGCTGGCATTATCACTGCTTTATGGTTGACAAATGGGTTCAG
PB1 Δ631–635FwGCAACCCACTGAACCCAGACATTGAATCAGTGAACAATGCA
RvTGCATTGTTCACTGATTCAATGTCTGGGTTCAGTGGGTTGC
1280 NA (vRNA)FwTGGACTAGTGGGAGCATCAT
NA160 (mRNA/cRNA)RvTCCAGTATGGTTTTGATTTCC
5S100RvTCCCAGGCGGTCTCCCATCC
  • a Fw, forward; Rv, reverse.