TABLE 7.

LCMV-GP-pseudotyped vectors transduce primary human glioma cellsa

Target cellsLentiviral pseudotypes Oncoretroviral pseudotypes
Vector pseudotype (TU on 293T cells)Transduced glioma cellsbGlioma cellscRatio (%)dVector pseudotype (TU on 293T cells)Transduced glioma cellsbGlioma cellscRatio (%)d
GlioblastomaLCMV-GP (103)3.610.634LCMV-GP (5 × 103)0.35.55
A-MLVenv (103)0.9713A-MLVenv (6 × 103)0.24.84
VSV-G (5 × 103)5.27.668VSV-G (3 × 103)0.78.98
OligodendrogliomaLCMV-GP (103)2.8694LCMV-GP (5 × 103)1552
A-MLVenv (103)0.7382A-MLVenv (6 × 103)0.4381
VSV-G (5 × 103)136420VSV-G (3 × 103)0.4640.6
  • a Human primary glioblastoma or oligodendroglioma cells were transduced with 1 × 103 to 6 × 103 eGFP TU of LCMV-GP-, A-MLVenv-, or VSV-G-pseudotyped oncoretroviral or lentiviral vectors. Cells were analyzed by fluorescence microscopy after immunostaining with monoclonal anti-eGFP antibody and polyclonal anti-GFAP antibody (glia antigen). Results are the mean cell numbers from 10 random camera fields.

  • b Mean cell numbers of eGFP+ GFAP+ double-positive transduced glioma cells from 10 random camera fields.

  • c Mean cell numbers of GFAP+ glioma cells from 10 random camera fields.

  • d Percent eGFP+ GFAP+ double-positive cells within the total number of GFAP+ glioma cells.