TABLE 1.

Production of LCMV-GP-pseudotyped oncoretroviral and lentiviral vectors

Transfected plasmidsPseudotype titer [TU/ml] (± SD)a
RV + MLVgagpol + LCMV GP1.3 (± 1.1) × 105
RV + LCMV GP<102 b
RV + MLVgagpol<102
RV<102
LV + HIVgagpol + HIVrev + LCMV GP2.8 (± 2.3) × 105
LV + LVgagpol+ HIVrev + LCMV GP<102
LV + LVgagpol + LCMV GP<102
LV + LCMV GP<102
LV<102
  • a Different combinations of LCMV-GP- and MLV-based oncoretroviral (RV) or HIV-based lentiviral (LV) packaging plasmids were cotransfected into 293T cells. Cell supernatants were analyzed by end-point dilutions on TE671 cells. Results are the mean vector titers and standard deviations of three experiments.

  • b Vector titer was lower than the detection limit.