TABLE 2.

Determination of infectious HCMV in supernatants of transfected cultures

Construct(s)Single green fluorescent cellsaGreen fluorescent miniplaquesbIE antigen in single cells after inoculation of HFFcMultiplication in HFFd
CMV GFP BAC++++
HCMV-BACΔgB + pRC/CMVgB+++
HCMV-BACΔgB + pRC/CMVSigDelgB+
HCMV-BACΔgB+
pRC/CMVgB
  • a Indicative of successful transfection of replication-deficient HCMV-BACΔgB.

  • b indicative of successful phenotypic complementation of replication-deficient HCMV-BACΔgB for one round of viral replication.

  • c Indicative of release of phenotypically complemented replication-deficient HCMV-BACΔgB competent to abortively infect single cells (10 to 50 HCMV -IE-positive nuclei/ml of supernatant).

  • d Indicative of replication-competent HCMV.