TABLE 1.

PCR primers used in this study

PrimerSequenceaRestriction site(s)SourcePositionsOrientationb
GFP.CN-F5′-ATAAGAATTC CAGATCCGCTAGCGCTACCG-3 EcoRIpEGFP-N1574–603+
EGFP.N1-R5′-TATA TCGCGA C TCATGA ACTTGTACAGCTCGTCC-3′ NruI, BspHIpEGFP-N11380–1403
R32.N-F5′-TCGCAGATCTCCCGCCAGCCC-3′RCMV genome25535–25555+
R32.N-R5′-TATA CCATGG CGGTCCCGTCTCCGTC-3′ NcoIRCMV genome26253–26280
R33.N-F5′-TAAT CCATGGACGTGCTGCTGGGGAC-3′ NcoIRCMV genome26271–26290+
R33.N-R5′-GGTTCGTCATGTACAGGGTCGGCGTC-3′RCMV genome26474–26499
R33.C-F5′-TCCTGGTGGTGCAGACGCCGTTC-3′RCMV genome27025–27047+
R33.C-R5′-CG TCTAGA CAGTTCGGCGGCGGAC-3′ XbaIRCMV genome27415–27438
R34.C-F5′-ACTG TCTAGA CGGCAGATCGCCGCC-3′ XbaIRCMV genome27427–27451+
R34.C-R5′-GCTTCTCCGTCTCCCCGCGCC-3′RCMV genome28024–28044
  • a Underlined sequences indicate restriction enzyme recognition sites; sequences in boldface type differ from that of the original sequence as described in the columns for source and positions.

  • b +, orientation of the sequence is identical to the published sequence between the indicated positions; −, sequence is complementary to the published sequence between the indicated positions.