TABLE 3.

LCMV-GP-pseudotyped oncoretroviral and lentiviral vectors

Vector typeMean pseudotype titer [TU/ml] (± SD)a
LCMV-GPVSV-GA-MLVenv
RV4.0 (± 2.8) × 105 2.5 (± 2) × 105 12 (± 9) × 105
LV4.4 (± 3.7) × 105 17 (± 4.1) × 105 4.1 (± 1.9) × 105
  • a Viral envelope proteins LCMV-GP(WE-HPI), VSV-G, or A-ML Venv were coexpressed with MLV-based oncoretroviral (RV) or HIV-based lentiviral (LV) packaging plasmids. Titers of oncoretroviral pseudotypes were determined on TE671 cells, and titers of lentiviral pseudotypes were determined on 293T cells. Results are the mean vector titers and standard deviations of at least three independent experiments.