Table 2.

Concentration of MLV(Ebola) by centrifugation

ExptVirus titer (IU/ml)aFold concnTotal virus (IU)% Recovery
PreconcnPostconcnPreconcnPostconcn
15.9 × 104 5.4 × 106 784.6 × 106 3.8 × 106 82.6
21.1 × 105 6.2 × 106 707.7 × 106 6.2 × 106 80.5
  • a Titers of the concentrated MLV(Ebola) were determined on 293T cells. Preconcentration titer refers to viral supernatant left overnight at 4°C before being used for infection. Postconcentration titer refers to viral supernatant centrifuged at 25,000 rpm in an SW28 rotor for 90 min and resuspended in TNE overnight at 4°C before being used for infection.