Table 2.

Quantitative measures of lung immunopathology in primed mice after RSV challengea

Priming regimen% Eosinophil in BAL fluidbConcn (mean ± SEM;n = 5)
IL-5 (pg/ml)cIFN-γ (ng/ml)
VSC80.0 ± 0.010.4 ± 2.117.8 ± 3.8
VSC8 + G8.3 ± 3.721.9 ± 3.55.1 ± 0.3
vvWT G5.9 ± 2.622.3 ± 5.110.5 ± 2.9
vvM487.6 ± 3.4104.4 ± 5.427.5 ± 4.4
vvM48I1.9 ± 0.915.1 ± 6.531.4 ± 2.5
vvM48I + G1.3 ± 0.664.0 ± 3.415.7 ± 2.7
  • a The mice were primed and challenged as for Fig. 3. Percentage of eosinophils in BAL fluid was determined by counting Diff-Quik-stained smears. Concentrations of IL-5 and IFN-γ were measured in day 4 (shown) and day 8 (not shown) lung supernatants by ELISA. Limits of detection are 10 pg/ml for IL-5 and 50 pg/ml for IFN-γ.

  • b Differences between VSC8, vvWT G, and vvM48 were not statistically significant by Student’s t test, nor were the differences between vvM48I and vvM48 plus G. However, the differences between the mice primed with vvM48I (or vvM48I plus G) and the mice primed with VSC8 plus G, vvWT G, or vvM48 were significant (P < 0.05 for all comparisons).

  • c Statistically significant differences were found as follows: vvM48I compared to vvM48I plus G (P = 0.01), and vvM48 compared to all other groups (P < 0.025). Differences between VSC8 and VSC8 plus G, between vvWT G and vvM48I, and between vvWT G and VSC8 plus G were not significant.