Table 2.

Tumorigenicity of Rat-1 cells transfected with the HCV-K core gene

CloneNo. of foci formedaColony formation in soft agar assay (%)bSize of tumors on nude mice (mm)
pCI-neo00.05 ± 0.03NDc
pCI-neo-core K(H)d 124 ± 613.19 ± 0.576.5 ± 0.5
pCI-neo-core K(L)d 3 ± 13.20 ± 0.084.5 ± 0.5
H-ras 231 ± 722.96 ± 0.78NTe
  • a A total of 5 × 104cells of each clone were mixed with Rat-1 control cells at a ratio of 1:10 and incubated for 6 days.

  • b The data is expressed as the percentage of colonies containing more than 200 cells 2 weeks after plating. Colony numbers are means of three independent experiments.

  • c ND, not detected.

  • d pCI-neo-core K(H) clones express the HCV-K core protein at higher level than pCI-neo-core K(L) clones. The data is represented as the mean of the results for three independent clones.

  • e NT, not tested.