TABLE 1.

Plasmids and viruses used

Plasmid or virusExon 12-kb LATExon 2 (kb)Apoptosis protectiona
Size (kb)Stability
Plasmids
    pcDNA3NoneNo
    ΔCMV(Pst-Mlu) (promoterless)Complete2.0Stable0.23No
    pcDNA3.Pst-MluComplete2.0Stable0.23+++
    pΔXcmComplete1.2 (3 deletion)Stable0.23++
    pConsComplete2.0Unstable0.23++
    pΔBstE3′ deletionNone (5′ deletion)0.23No
    pΔStyMiddle deletion2.0Stable0.23No
    pΔSty + pΔXcmComplete2.0Stable0.23+++
Viruses
    17Complete2.0Stable∼7+++
    17N/HNoneNone (5′ deletion)∼7No
    ΔStyMiddle deletion2.0Stable∼7+++
  • a ++, 20 to 30% protection; +++, 30 to 50% protection.