TABLE 1.

SWPV ORFs

ORFPosition (length, codons)SPV accession no.aBest matchPredicted structure and/or functiondPromoter typeeLSDVYLDVMYXVV
SpeciesAccession no.aBLASTP2 score% Id.jLength (aa)cORFf% Id.jORFg% Id.jORFh% Id.jORFi% Id.j
SPV001736-287 (150)LSDVAF32552849862145A52R family proteinLSDV001626L41M003.145B15R41
SPV0021323-814 (170)ShPVbP1838819539113TMLSDV00239M003.229
SPV0032452-1433 (340)P32231YLDVAJ29356853938335MHC class 1 α chain-like protein, TME2L38
SPV0042772-2497 (92)P32230LSDVAF3255282174691LSDV00446150R42M004.144
SPV0053630-2824 (269)Q08520HumanU4598349737267GPCR, TMLSDV011387L/145R36
SPV0065285-3696 (530)P32228RFVAF17072282433523Kelch-like proteinLSDV15127140R28M008L34A55R29
SPV0076038-5331 (236)P32227YLDVAJ29356844443217A52R family proteinLSDV009293L43K7R35
SPV0086885-6064 (274)P32226LSDVAF32552843537257IFN-γ receptor, SP, TMELSDV00837M00725B8R28
SPV0097385-6921 (155)P32225LSDVAF32552830935150LAP/PHD-finger protein, TMLSDV010355L34M153R36
SPV0107705-7448 (86)P32224LSDVAF3255282484985elF2α-like PKR inhibitorELSDV0144912L48M156R40K3L44
SPV0118146-7745 (134)P32223LSDVAF32552830844129IL-18 binding proteinLSDV0154414L42
SPV0128672-8172 (167)P32222LSDVAF32552816834171Integral membrane protein, apoptosis regulatorELSDV0173416L31M011L27
SPV0139153-8728 (142)P32208LSDVAF32552850568141dUTPaseLSDV0186817L65M012L59F2L58
SPV0149576-9205 (124)P32221YLDVAJ29356824338124E18L38M013L36
SPV01511205-9604 (534)P32206LSDVAF3255281,10040564Kelch-like proteinLSDV0194019L38M014L39F3L28
SPV01612243-11284 (320)P32209VARU183381,34177320Ribonucleotide reductase (small subunit) TMELSDV0207820L78M015L74F4L76
SPV01712529-12272 (86)P32220LSDVAF3255281793886SP, TMLSDV0213821L33M016L28
SPV01812789-12571 (73)P32219
SPV01913012-12803 (70)P32218E
SPV02013142-12942 (67)P32217SP, TM
SPV02114156-13512 (215)P32207LSDVAF32552875464216TMLLSDV0246424L50M019L52F9L45
SPV02215462-14143 (440)P32216LSDVAF3255281,99680435Ser/Thr protein kinase, virus assemblyLLSDV0258025L76M020L77F10L72
SPV02316562-15486 (359)VVAF09568944831351ELSDV02634F11L31
SPV02418530-16596 (645)LSDVAF3255281,54651631EEV maturation, TMELSDV02751A26L49M021L46F12L39
SPV02519678-18566 (371)AJ249689ShPVAF1995941,49976368EEV envelope protein, virus assemblyLSDV0287527L74M022L73F13L56
SPV02619915-19727 (63)AL109739723053
SPV02720589-20146 (148)LSDVAF32552851466148LSDV0296629L62M024L49F15L60
SPV02821311-20661 (217)LSDVAF32552850244218LSDV0304430L39M025L36F16L42
SPV02921372-21680 (103)YLDVAJ29356838872100DNA binding virion core phosphoproteinLLSDV0317131R72M026L70F17R62
SPV03023092-21683 (470)LSDVAF3255281,87775474Poly(A) polymerase PAPLSDV0327532L71M027L74E1L65
SPV03125324-23129 (732)LSDVAF3255282,08955714LSDV0335533L50M028L53E2L44
SPV03225909-25379 (177)YLDVAJ29356847150180PKR inhibitor, host rangeELSDV0344934L50M029L50E3L34
SPV03326552-25938 (205)LSDVAF32552879571199RNA polymerase subunit RPO30ELSDV0367135L70M030R68E4L68
SPV03426660-28363 (568)LSDVAF3255282,26773568E/LLSDV0377337R68M032R67E6R60
SPV03528370-29155 (262)LSDVAF3255281,14780265ER-localized protein, TMLSDV0388038R74M033R81E8R66
SPV03632190-29167 (1008)MYXAF1707264,123751,008DNA polymeraseELSDV0397539L68M034L75E9L66
SPV03732223-32510 (96)MYXAF1707263826796Potential redox protein, virus assemblyLSDV0406940R71M035R67E10R66
SPV03834587-32557 (677)MYXAF1707261,52142678ELSDV0424342L43M036L42O1L34
SPV03935664-34726 (313)LSDVAF3255281,18772314DNA binding virion core protein, virus assemblyLLSDV0437243L67M038L70I1L66
SPV04035892-35668 (75)RFVAF1707222025373TMLLSDV0445244L53M039L5712L59
SPV04136756-35896 (287)LSDVAF32552891565276DNA binding phosphoproteinLSDV0456545L61M040L60I3L53
SPV04239041-36771 (757)VARP329842,72766751Ribonucleotide reductase (large chain)I4L66
SPV04339324-39091 (78)LSDVAF3255282645678IMV membrane protein, SP, TMLLSDV0465646L55M041L51I5L31
SPV04440497-39343 (385)LSDVAF3255281,18157394TMLSDV0475747L50M042L52I6L51
SPV04541791-40493 (433)LSDVAF3255281,79275433Virion core proteinLLSDV0487548L74M043L74I7L68
SPV04641797-43839 (681)LSDVAF3255282,31564681NPH-II, RNA helicaseLSDV0496449R61M044R62I8R58
SPV04745620-43842 (593)LSDVAF3255282,15867595Metalloprotease, virion morphogenesisLLSDV0506750L63M045L64G1L57
SPV04845946-46641 (232)LSDVAF32552876863222Putative transcriptional elongation factorLSDV0516352R48M047R55G2R44
SPV04945952-45620 (111)RFVAF17072238463111TMLLSDV0525851L54M046L63G3L47
SPV05046958-46584 (125)LSDVAF32552848870124Glutaredoxin 2, virion morphogenesis,LLSDV0537053L64M048L64G4L43
SPV05146963-48279 (439)LSDVAF3255281,48663438LSDV0546354R54M049R52G5R46
SPV05248285-48473 (63)LSDVAF3255282868763RNA polymerase subunit RPO7ELSDV0558755R84M050R82G5.5R77
SPV05348476-48994 (173)LSDVAF32552853960169TMLSDV0566056R55M051R59G6R43
SPV05450144-49002 (381)LSDVAF3255281,22765378Virion core protein, TMLLSDV0576557L60M052L54G7L53
SPV05550174-50953 (260)LSDVAF3255281,24591260Late transcription factor VLTF-1, TMLSDV0589158R88M053R85G8R83
SPV05650949-52016 (356)LSDVAF3255281,03858336Myristylated proteinLSDV0595859R56M054R52G9R45
SPV05752020-52766 (249)LSDVAF3255281,11185245Myristylated IMV envelope protein, TMLLSDV0608560R81M055R75L1R67
SPV05852799-53080 (94)LSDVAF3255282425085TMELSDV0615061R40M056R29L2R31
SPV05954025-53066 (320)LSDVAF3255281,20570320LLSDV0627062L65M057R65L3L49
SPV06054051-54809 (253)LSDVAF3255281,07881253DNA-binding virion core protein VP8LLSDV0638163R77M058R79L4R62
SPV06154824-55210 (129)LSDVAF32552841264126TMLLSDV0646464R54M059R52L5R50
SPV06255167-55610 (148)P23332ShPVP1974645160148LLSDV0656065R57M060R53J1R53
SPV06355625-56167 (181)M64000ShPVP1660064068175Thymidine kinase, SPLSDV0666866R66M061R67J2R64
SPV06456225-56779 (185)P23333ShPVP1974746748187Host range proteinELSDV0674867R45M062R41C7L39
SPV06556835-57833 (333)RFVAF1707221,43079333Poly(A) polymerase PAPsLSDV0687768R72M065R78J3R72
SPV06657751-58305 (185)LSDVAF32552881681185RNA polymerase subunit RPO22LSDV0698169R75M066R72J4R66
SPV06758715-58314 (134)RFVAF17072254168132LSDV0706770L65M067L67J5L60
SPV06858782-62636 (1285)LSDVAF3255285,865841,285RNA polymerase subunit RPO147LSDV0718471R84M068R84J6R80
SPV06963169-62651 (173)ShPVAF12451771878171Protein tyrosine phosphatase, virus assemblyLLSDV0727872L72M069L73H1L61
SPV07063184-63753 (190)ShPVAF12451778973190TMLSDV0737373R67M070R71H2R65
SPV07164742-63771 (324)LSDVAF3255281,07561320IMV envelope protein p35, TMLLSDV0746174L54M071L54H3L40
SPV07267148-64746 (801)LSDVAF3255283,37878801RNA polymerase-associated protein RAP94LLSDV0757875L76M072L75H4L69
SPV07367315-67857 (181)YLDVAJ29356843650181Late transcription factor VLTF-4ELSDV0764676R50M073R48H5R37
SPV07467896-68855 (320)LSDVAF3255281,20472315DNA topoisomeraseLSDV0777277R61M074R66H6R61
SPV07568867-69313 (149)LSDVAF32552849968145LLSDV0786878R53M075R55H7R43
SPV07669320-71839 (840)LSDVAF3255283,32072842mRNA capping enzyme (large subunit)LSDV0797279R70M076R71D1R65
SPV07772244-71807 (146)YLDVAJ29356835547149Virion proteinLSDV0804680L47M077L46D2L40
SPV07872249-72980 (244)LSDVAF32552846939241Virion proteinLSDV0813981R37M078R37D3R34
SPV07972980-73630 (217)RFVAF17012692672216Uracil DNA glycosylase, DNA replicationLSDV0827382R73M079R72D4R69
SPV08073663-76020 (786)LSDVAF3255283,32476786NTPase, DNA replication, TMLLSDV0837683R77M080R76D5R68
SPV08176020-77924 (635)LSDVAF3255283,00490635Early transcription factor VETFs, TMLLSDV0849084R87M081R87D6R81
SPV08277952-78434 (161)LSDVAF32552873181160RNA polymerase subunit RPO18LLSDV0858185R75M082R77D7R63
SPV08378507-79145 (213)LSDVAF32552874469213mutT motifELSDV0866986R64M084R61D9R57
SPV08479145-79870 (242)RFVP3209770956237mutT motif, gene expression regulatorLLSDV0875587R61M085R59D10R45
SPV08581766-79871 (632)LSDVAF3255282,60677632NPH-I, transcription termination factorLLSDV0887788L73M086L74D11L69
SPV08682647-81787 (287)Q08512LSDVAF3255281,25980287mRNA capping enzyme (small subunit), VITFE/LLSDV0898089L82M087L76D12L77
SPV08784330-82678 (551)Q08517YLDVAJ2935682,34778550Rifampin resistance protein, IMV assemblyLSDV0907790L78M088L76D13L70
SPV08884807-84358 (150)LSDVAF32552852965150Late transcription factor VLTF-2LLSDV0916591L62M089L64A1L57
SPV08985531-84860 (224)LSDVAF3255281,03987225Late transcription factor VLTF-3LSDV0928792L84M090L85A2L80
SPV09085755-85531 (75)LSDVAF3255282977275LLSDV0937293L56M091L668.9 kDa*50
SPV09187720-85765 (652)LSDVAF3255282,73678656Virion core protein P4bLLSDV0947894L78M092L75A3L67
SPV09288230-87769 (154)LSDVAF32552840752158Virion core protein, virion morphogenesisLLSDV0955295L38M093L32A4L27
SPV09388271-88756 (162)LSDVAF32552854265170RNA polymerase subunit RPO19LLSDV0966596R57M094R65A5R59
SPV09489877-88759 (373)MYXAF1707261,52977372LSDV0977897L70M095L77A6L56
SPV09592035-89903 (711)LSDVAF3255283,02780714Early transcription factor VETFLLSDV0988098L77M096L80A7L71
SPV09692097-92972 (292)LSDVAF3255281,09074288Intermediate transcription factor VITF-3LSDV0997499R68M097R70A8R62
SPV09793226-92975 (84)LSDVAF3255283528478IMV membrane protein, morphogenesis, SP, TMLLSDV10084100L83M098L80A9L69
SPV09895941-93230 (904)LSDVAF3255283,49172904Virion core protein P4aLLSDV10172101L68M099L65A10L51
SPV09995956-96888 (311)YMTVAB0158851,23776314LLSDV10275102R77M100R75A11R53
SPV10097433-96900 (178)MYXAF17072643566153Virion core proteinLLSDV10357103L62M101L66A12L52
SPV10197657-97454 (68)LSDVAF3255281905768IMV membrane protein, TMLLSDV10457104L54M102L48A13L33
SPV10298006-97725 (94)YLDVAJ2935684208794IMV membrane protein, SP, TMLLSDV10582105L87M103L70A14L55
SPV10398184-98026 (53)YLDVAJ2935682358452Virulence factor, SPLSDV10675106L84M104L76A14.556
SPV10498458-98177 (94)YMTVAB0158852635194LLSDV10749107L46M105L50A15L47
SPV10599581-98445 (379)LSDVAF3255281,38766379Putative myristylated membrane protein, TMLLSDV10866108L60M106L60A16L52
SPV106100192-99611 (194)YLDVAJ29356869269194Phosphorylated IMV membrane protein, TMLLSDV10964109L69M107L54A17L42
SPV107100207-101643 (479)LSDVAF3255281,62365480DNA helicase, transcriptional elongation, TMLSDV11065110R63M108R61A18R57
SPV108101845-101630 (72)LSDVAF3255282927672LLSDV11176111L72M109L76A19L63
SPV109102189-103472 (428)LSDVAF3255281,26657427DNA polymerase proces- sivity factorELSDV11257113R48M111R54A20R46
SPV110102190-101849 (114)MYXAF17072639164114TMLSDV11362112L62M110L64A21L59
SPV111103453-103950 (166)VARX7626865371164DNA processingELSDV11464114R71M112R61A22R71
SPV112103979-105124 (382)LSDVAF3255281,27466383Intermediate transcription factor VITF-3LSDV11566115R63M113R62A23R59
SPV113105129-108614 (1162)LSDVAF3255285,459891,156RNA polymerase subunit RPO132LSDV11689116R87M114R86A24R82
SPV114109061-108615 (149)LSDVAF32552834548144Fusion protein, virus assemblyLLSDV11748117L41M115L52A27L45
SPV115109485-109054 (144)ShPVP1671853971139SP, TMLLSDV11871118L64M116L65A28L50
SPV116110404-109499 (302)MYXAF1707261,13765302RNA polymerase subunit RPO35ELSDV11968119L63M117L65A29L59
SPV117110597-110376 (74)YLDVAJ2935682206373Virion protein, maturationLSDV12061120L63M118L57A30L51
SPV118111542-110787 (252)LSDVAF3255281,10982252DNA packaging, virus assemblyLSDV12182121L79M120L80A32L58
SPV119111706-112260 (185)LSDVAF32552835644179EEV glycoprotein, TMLSDV12244122R41M121R38A33R30
SPV120112290-112796 (169)YLDVAJ29356864464169EEV proteinLSDV12358123R64M122R60A34R50
SPV121112809-113363 (185)LSDVAF32552844445177ELSDV12445124R46M123R39A35R36
SPV122113341-114282 (314)LSDVAF32552878553288TMLSDV12553125R39M124R44
SPV123114313-114909 (199)YLDVAJ29356824440191EEV glycoprotein, TMLSDV12636126R40M125R28A36R25
SPV124114913-115752 (280)LSDVAF32552875251271TMLLSDV12751127R40M126R44A37R28
SPV125116661-115783 (293)MYXAF17072657638281CD47-like protein, SP, TMLSDV12831128L30M128L38A38L21
SPV126116801-117178 (126)LSDVAF32552812833112LSDV12933M130R28
SPV127117250-117486 (79a)LSDVAF3255281805475LSDV13054132R40
SPV128118520-117489 (344)YLDVAJ29356895953343Hydroxysteroid dehydrogenase-like proteinE133L53A44L45
SPV129118565-119053 (163)LSDVAF32552855064159Superoxide dismutase-like proteinLSDV13164M131R59A45R31
SPV130119089-120765 (559)LSDVAF3255281,91263558DNA ligaseLSDV13363M133R61A50R50
SPV131120870-126746 (1959)LSDVAF3255285,419562,007VAR B22R homologue, TMLSDV13456135R50M134R50
SPV132126727-127758 (344)YLDVAJ29356840831346IFN-α/β binding protein, SPLSDV13532136R31M135R29B18R28
SPV133127790-128326 (179)LSDVAF32552835846156A52R family proteinLSDV13646137R30M136R42K7R25
SPV134128372-129355 (328)LSDVAF32552888250335ELSDV13750138R39M137R39A51R33
SPV135129411-129974 (188)MYXAF17072653958187A52R family proteinLSDV13635139R45M139R58A52R38
SPV136129994-131715 (574)YLDVAJ2935681,31744564Kelch-like protein, TMLSDV15129140R44M140R43A55R31
SPV137131727-132653 (309)LSDVAF3255281,06965297Ser/Thr protein kinase, DNA replicationLSDV13965142R55M142R61B1R46
SPV138132666-133403 (246)RFVL2634259749226N1R/p28-like host range RING finger proteinLSDV14046143R41M143R49
SPV139133451-134146 (232)MYXAF17072649745215EEV host range protein, TMLSDV14142144R44M144R45C3L34
SPV140134184-135080 (299)LSDVAF32552888758296Tyrosine protein kinase-like proteinLSDV14358M147R48
SPV141135128-137032 (635)LSDVAF3255281,51546627Ankyrin repeat proteinLSDV14546146R29M148R38B4R26
SPV142137100-138554 (485)RFVAF1707221,18346484Ankyrin repeat protein, TMLSDV14743146R38M149R44B4R24
SPV143138662-139951 (430)LSDVAF32552877839426Ankyrin repeat proteinELSDV14839147R33M148R29B4R22
SPV144140003-141481 (493)LSDVAF32552877634492Ankyrin repeat proteinELSDV15234148R28M00530B4R28
SPV145141494-142453 (320)L21931LSDVAF32552860041334SerpinELSDV14941149R38M151R33C12L31
SPV146142522-143631 (370)Q08520HumanAY01637058937343GPCR, TMLSDV01137145R/7L38M139R29
SPV147143683-143958 (92)P32230LSDVAF3255282174691LSDV15346150R42M004.144
SPV148144003-145022 (340)P32231YLDVAJ29356853938335MHC class I α chain-like protein, SP, TME2L38
SPV149145132-145641 (170)ShPVP1838819539113TMLSDV15539M003.229
SPV150145719-146168 (150)LSDVAF32552849862145A52R family proteinLSDV156656L41M003.145B15R41
  • a Accession numbers are from the GenBank or SwissProtein database.

  • b ShPV, sheeppox virus.

  • c aa, amino acids.

  • d Function was deduced either from the degree of similarity to known genes or from the presence of Prosite signatures. TM, a Z score of >1.96 was used for the prediction of transmembrane (TM) domains with the MEMSAT computer program (21). SP, N-terminal signal peptide (Z score of >3.5 within 40 amino acids of the N terminus determined using the SIGCLEAVE computer program [ftp://ftp.ebi.ac.uk/pub/software/unix/EMBOSS/] [43]).

  • e Putative promoters (E, early; I, intermediate; L, late) were identified as previously described (2).

  • f Best-matching ORF from the LSDV genome (accession no. AF325528).

  • g Best-matching ORF from the YLDV genome (accession no. AJ293568).

  • h Best-matching ORF from the MYX genome (accession no. AF170726).

  • i Best-matching ORF from the VV Copenhagen genome (accession no. M35027), with the exception of the 8.9-kDa protein (*) (accession no. P07608), which corresponds to the VV Ankara strain.

  • j Percentage of amino acid identity in the BLASTP2 analysis.