Table 2.

Spontaneous 3′-NCR deletions detected in a panel of recombinant TBE virus mutants

VirusaNo. of passagesbNo. of deletions in suckling micecAdult miced
No. testedeNo. of deletionsf
R-11020
R-21000
R-31020
R-41010
R-51000
R-61020
R-71082 (10394–10604, 10441–10537)
R-81000
R-91000
R-101010
R-115011 (10450–10620)
R-125011 (10494–10596)
R-135021 (10495–10574)
R-1452 (10458–10689, 10458–10811)22 (10504–10794, 10453–10603)
R-155010
R-1651 (10485–10552)11 (10454–10592)
R-179000
Total3248
  • a Recombinant TBE virus Neudoerfl mutants bearing specific mutations in regions of the genome other than the 3′ NCR, consecutively numbered.

  • b Number of BHK cell culture passages (at low MOI) prior to the generation of suckling-mouse brain stock suspensions.

  • c Number of deletion mutants found in suckling-mouse brain stock suspensions; boundaries of deletions (positions of the first and last deleted nucleotides) are in parentheses.

  • d Virus isolated from the brains of adult mice after infection with the suckling-mouse brain stock suspensions.

  • e Number of viral isolates tested.

  • f Number of deletion mutants found; boundaries of deletions in parentheses.