Table 1.

Binding sites for NCa

OligonucleotideNo. of NC binding sites
 d(TGTGT)1
 d(GTGTG)1
 d(TG)4 1
 d(TG)5 2
 d(TG)10 4
  • a Analysis of the washout portion of SPR experiments shows that there are two independent systems for the binding of NC to short oligonucleotides. The more stable system achieves saturation but the less stable system does not. Stoichiometry for binding was determined by extrapolation of the shallow portion of the washout curve, where the more stable binding system predominates, by using a simple exponential function to find the number of apparent binding sites. This number was then rounded to the nearest integer.