TABLE 2

Detection of PrPSc in lymphoid tissues by antigen capture EIA and RT-QuIC assay

Treatment group and animal no.Incubation period (mpi)Overall resultRPLNbTonsilMLNc
EIA resultRT-QuIC resultEIA resultRT-QuIC resultEIA resultRT-QuIC result
Noninoculated controls
    56
    973NAaNA
i.c. inoculated market wt
    126++
    146+++
    156++++
    166++++
    186++
    196
i.c. inoculated aged
    2230++
    2442+NANA+NANA
    2545++
    2656+++NANA
    2764NANANANA
    2873+NANA++
    2973++
Orally inoculated market wt
    306++
    326+++
    346
    366+++
    376++++
    386+++
Orally inoculated aged
    3919++
    4141+++
    4345+NA+NANA
    4455+NANA+
    4564NANANA
    4872NANA
  • a NA, sample not available.

  • b RPLN, retropharyngeal lymph node.

  • c MLN, mesenteric lymph node.