TABLE 2

Relative abundance of posttranslational modifications to mitochondrial proteins in hamster prion-infected Tg7 mice

Protein nameProtein identifierPTMaFold ΔbP valuec
28S ribosomal protein S34Q9JIK9Deamidation (NQ)24.90.002
NADH dehydrogenase 1 α subcomplex subunit 9Q9DC69Pyro-glu from Q5.00.02
Isoleucyl-tRNA synthetaseQ8BIJ6Pyro-glu from Q4.10.02
Sideroflexin-1Q99JR1Acetylation (N-term)2.10.01
Voltage-dependent anion-selective channel protein 2Q60930Acetylation (N-term)1.70.003
Mitochondrial inner membrane proteinQ8CAQ8Pyro-glu from Q1.70.01
Cytochrome b-c1 complex subunit 2Q9DB77Deamidation (NQ)−1.30.003
Sideroflexin 1Q99JR1Oxidation (M)−1.30.03
NADH dehydrogenase 1 α subcomplex subunit 12Q7TMF3Acetylation (N-term)−1.40.003
Glutamate dehydrogenase 1P26443Deamidation (NQ)−1.60.005
Cytochrome c1 heme proteinQ9D0M3Deamidation (NQ)−1.60.02
ATP synthase subunit αQ03265Deamidation (NQ)−1.80.03
NADH dehydrogenase iron-sulfur protein 3Q9DCT2Pyro-glu from Q−1.90.005
Ca2+-binding mitochondrial carrier protein Aralar1Q8BH59Deamidation (NQ)−1.90.04
ATP synthase subunit αQ03265Sodium adduct−2.20.03
Ca2+-binding mitochondrial carrier protein Aralar1Q8BH59Deamidation (NQ)−2.40.03
ATP synthase subunit αQ03265Formylation−2.40.003
NADH dehydrogenase iron-sulfur protein 2Q91WD5Oxidation (M)−2.50.01
Mitochondrial inner membrane proteinQ8CAQ8Pyro-glu from Q−2.70.03
Cytochrome c oxidase subunit 4 isoform 1P19783Deamidation (NQ)−3.30.04
  • a Posttranslational modification (PTM) identified on a single peptide from the protein identified. The amino acids altered are indicated. A protein occurring multiple times in the list indicates that more than one peptide with a PTM was identified. SpCs were derived using the PEAKS Studio version 8 software package. M, methionine; Q, glutamine; NQ, asparagine and glutamine; N-term, N-terminal amino group of peptide.

  • b Fold change was calculated from the average normalized SpC values from each group and indicates the change in relative abundance in Tg7Sc mice from that in Tg7NBH mice. If either group had an average SpC of 0, the value of 1 was assigned to obtain an accurate fold change.

  • c P values were calculated using the Student t test to compare SpC in Tg7NBH mice to SpC in Tg7Sc mice.