Table 4.

Inoculation of suckling mice and 5-week-old mice

VirusSuckling mice (intracranial inoculation)5-week-old mice (subcutaneous inoculation)
LD50 (PFU)Mean survival time (days) aftera:LD50(PFU)ID50(PFU)LD50/ID50
1 PFUb0.1 PFUb
Neudoerfl10−1.7 7.2 (±1.5)8.2 (±1.5)100.9 10−0.2 101.1
R-Nd/3′Δ1079510−2.5 7.7 (±1.4)8.5 (±0.9)101.0 10−0.4 101.4
R-Nd/3′Δ1082610−2.3 11.0 (±0.8)10.7 (±1.7)104.9 100.4 104.5
R-Nd/3′Δ1084710−1.9 11.1 (±0.9)11.4 (±0.9)105.0 101.0 104.0
R-Nd/3′Δ1087010−2.1 12.5 (±2.5)11.9 (±1.5)>105.0 102.4 >102.6
R-Nd/3′Δ1091910−2.4 12.6 (±0.6)11.5 (±0.5)>105.0 100.5 >104.5
  • a In parentheses is the standard error of the mean, calculated as ς =Embedded Image

  • b Inoculation dose/mouse. For each virus and dose, litters of 7 to 12 mice were inoculated. No mice survived at these inoculation doses.