Table 4.

Quantitative transposition frequencies of mutants 2 and 5 and PCR reconstructions

StrainPlasmid descriptionG418r colonies/FOArcolonies% Transposition
1282Wild-type Tf1-neoAI 78/3,7962.05
1554Tf1-neoAI with IN frameshift3/5,0860.06
1836Tf1-neoAI with PR frameshift0/4,290<0.02
2234Mutant 5 (S749L)6/5,2520.11
5274Mutant 5; PCR reconstruction 16/3,3760.18
5278Mutant 5; PCR reconstruction 25/3,3310.15
2230Mutant 2 (P816S)7/5,7450.12
5282Mutant 2; PCR reconstruction 17/4,7070.15
5286Mutant 2; PCR reconstruction 25/2,3220.22