Table 1.

Detection of viral nucleic acids by in situ hybridization in tissues of mice infected by the i.p. or i.n. route

RouteOrgan In situ hybridizationa at indicated dpi
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i.n.Spleen++++++NDb
Lung++ND
Brain++ND
Spinal cord++ND
Kidney+ND
Peyer’s patches++ND
Lymph node+c +c ND
i.p.SpleenND+ND++++++++++++
LungNDND++++
BrainNDND+++++
Spinal cordNDNDNDNDNDNDNDNDNDND
KidneyNDND++++
Peyer’s patchesNDND++
Lymph nodeNDND+c +d +d
  • a +, viral nucleic acid was presented but very infrequent; ++, few high-power fields had viral nucleic acid; +++, most high-power fields had viral nucleic acid.

  • b ND, not done.

  • c Mandibular.

  • d Prefemoral.