TABLE 1

Changes in relative abundance of mitochondrial proteins in hamster prion-infected Tg7 mice

Protein nameProtein identifierTg7NBH SpCaTg7Sc SpCaFold ΔbP valuec
NADH-ubiquinone oxidoreductase chain 3P038990 ± 06.2 ± 118.5<0.001
Coiled-coil-helix-coiled-coil-helix domain-containing protein 1Q9CQA61.0 ± 1.76.4 ± 1.66.70.01
Mitofusin 1Q811U42.2 ± 2.07.2 ± 1.43.30.02
Glycine amidinotransferaseQ9D96428.3 ± 5.791.2 ± 28.83.20.02
Translocase subunit Tim8 AQ9WVA22.1 ± 1.96.1 ± 0.32.90.02
Monoacylglycerol lipase ABHD12Q99LR114.2 ± 5.127.2 ± 4.31.90.03
Sarcosine dehydrogenaseQ99LB77.5 ± 1.211.9 ± 1.41.60.01
Valyl-tRNA synthetaseQ3U2A810 ± 0.415 ± 2.71.50.03
NLR family member X1Q3TL4418.2 ± 2.125.4 ± 1.21.40.007
Mitoguardin 2Q8BK0311.7 ± 2.215.9 ± 0.81.40.04
28S ribosomal protein S9Q9D7N320.1 ± 2.125.2 ± 1.11.30.02
NADH dehydrogenase iron-sulfur protein 3Q9DCT2285.2 ± 15.9246.4 ± 14.8−1.20.04
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1Q3UV7036.3 ± 1.730.1 ± 1.6−1.20.009
Kynurenine-oxoglutarate transaminase 3Q71RI922.4 ± 2.217.6 ± 0.9−1.30.02
NADH dehydrogenase iron-sulfur protein 5Q99LY973.9 ± 4.551.6 ± 8.4−1.40.02
ProhibitinP67778256.6 ± 26.1176.8 ± 3.7−1.50.006
Citrate lyase subunit beta-like proteinQ8R4N036.3 ± 4.624.3 ± 2.7−1.50.02
Probable prolyl-tRNA synthetaseQ8CFI516.1 ± 1.710.4 ± 1.5−1.50.01
NADH dehydrogenase iron-sulfur protein 8Q8K3J166.2 ± 14.940.7 ± 5.1−1.60.05
Tryptophanyl-tRNA synthetaseQ9CYK112.0 ± 0.75.8 ± 2.4−2.10.01
ATP synthase subunit deltaQ9D3D918.7 ± 6.56.5 ± 3.7−2.90.05
Choline dehydrogenaseQ8BJ646.3 ± 1.31.2 ± 2.1−5.20.02
  • a Average number of peptides (spectral counts or SpC) ± standard deviation. The average is derived from LC-MS/MS analysis of mitochondria extracted from 3 individual mouse brains. SpCs were derived using the Byonic software package.

  • b Fold change was calculated from the average normalized SpC values from each group and indicates the change in relative abundance in Tg7Sc mice compared to Tg7NBH mice. If either group had an average SpC of 0, the value of 1 was assigned to obtain an accurate fold change.

  • c P values were calculated using the Student t test to compare SpC in Tg7NBH mice to SpC in Tg7Sc mice.