TABLE 1

Steady-state kinetic properties of N9 NAs

SubstrateNA proteinMean ± SE Km (μM)Mean ± SE kcat (s−1)kcat/Km (μM−1 s−1)
MUNANAHb+137.9 ± 12.890.1 ± 4.70.654
MUNANAHb134.2 ± 12.988.6 ± 4.70.660
6SLNHb+4,179 ± 6152.2 ± 0.20.000537
3SLNHb+810 ± 100159.5 ± 8.50.197
6SLNX-31a8,070 ± 61518.5 ± 0.90.00229
3SLNX-31a562.3 ± 2097.5 ± 1.50.173
FetuinHb+197.5 ± 37.371.6 ± 8.30.363
FetuinHb346.4 ± 95.673.0 ± 14.50.211
  • a The values were determined in a previous study (16).