RT Journal Article
SR Electronic
T1 Differential Type I Interferon Induction by Respiratory Syncytial Virus and Influenza A Virus In Vivo
JF Journal of Virology
JO J. Virol.
FD American Society for Microbiology
SP 9790
OP 9800
DO 10.1128/JVI.00530-07
VO 81
IS 18
A1 Jewell, Nancy A.
A1 Vaghefi, Negin
A1 Mertz, Sara E.
A1 Akter, Parvis
A1 Peebles, R. Stokes
A1 Bakaletz, Lauren O.
A1 Durbin, Russell K.
A1 Flaño, Emilio
A1 Durbin, Joan E.
YR 2007
UL http://jvi.asm.org/content/81/18/9790.abstract
AB Type I interferon (IFN) induction is an immediate response to virus infection, and very high levels of these cytokines are produced when the Toll-like receptors (TLRs) expressed at high levels by plasmacytoid dendritic cells (pDCs) are triggered by viral nucleic acids. Unlike many RNA viruses, respiratory syncytial virus (RSV) does not appear to activate pDCs through their TLRs and it is not clear how this difference affects IFN-α/β induction in vivo. In this study, we investigated type I IFN production triggered by RSV or influenza A virus infection of BALB/c mice and found that while both viruses induced IFN-α/β production by pDCs in vitro, only influenza virus infection could stimulate type I IFN synthesis by pDCs in vivo. In situ hybridization studies demonstrated that the infected respiratory epithelium was a major source of IFN-α/β in response to either infection, but in pDC-depleted animals only type I IFN induction by influenza virus was impaired.