RT Journal Article
SR Electronic
T1 In vitro infection of primary and retrovirus-infected human leukocytes by human foamy virus.
JF Journal of Virology
JO J. Virol.
FD American Society for Microbiology
SP 2774
OP 2780
VO 70
IS 5
A1 Mikovits, J A
A1 Hoffman, P M
A1 Rethwilm, A
A1 Ruscetti, F W
YR 1996
UL http://jvi.asm.org/content/70/5/2774.abstract
AB The infectivity of human foamy virus (HFV) was examined in primary and cultured human leukocytes. Cell-free infectious viral stocks of HFV were prepared from the human kidney cell line 293 transfected with an infectious molecular clone of HFV. HFV productively infects a variety of human myeloid and lymphoid cell lines. In addition, primary cell cultures enriched for human CD4+, monocytes and brain-derived microglial cells, were readily infected by HFV. Interestingly, while infected primary CD4+ lymphocytes and microglial cells showed marked cytopathology characteristic of foamy virus, HFV-infected monocyte-derived macrophages failed to show any cytopathology. In addition, marked cytotoxicity due to HFV infection was seen in both human T-cell leukemia virus type 1- and human immunodeficiency virus type 1-infected T-cell lines and in human immunodeficiency virus type 1-infected monocytoid cell lines. Thus, HFV infection produces differential cytopathology in a wide host range of primary human leukocytes and hematopoietic cell lines.