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Vaccines and Antiviral Agents

Inhibition of Human Adenovirus Replication by the Importin α/β1 Nuclear Import Inhibitor Ivermectin

Cason R. King, Tanner M. Tessier, Mackenzie J. Dodge, Jason B. Weinberg, Joe S. Mymryk
Lawrence Banks, Editor
Cason R. King
aDepartment of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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Tanner M. Tessier
aDepartment of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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Mackenzie J. Dodge
aDepartment of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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Jason B. Weinberg
bDepartment of Pediatrics, University of Michigan, Ann Arbor, Michigan, USA
cDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA
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Joe S. Mymryk
aDepartment of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
dDepartment of Oncology, University of Western Ontario, London, Ontario, Canada
eLondon Regional Cancer Program and Lawson Health Research Institute, London, Ontario, Canada
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Lawrence Banks
International Centre for Genetic Engineering and Biotechnology
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DOI: 10.1128/JVI.00710-20
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ABSTRACT

Human adenoviruses (HAdV) are ubiquitous within the human population and comprise a significant burden of respiratory illnesses worldwide. Pediatric and immunocompromised individuals are at particular risk for developing severe disease; however, no approved antiviral therapies specific to HAdV exist. Ivermectin is an FDA-approved broad-spectrum antiparasitic drug that also exhibits antiviral properties against a diverse range of viruses. Its proposed function is inhibiting the classical protein nuclear import pathway mediated by importin-α (Imp-α) and -β1 (Imp-β1). Many viruses, including HAdV, rely on this host pathway for transport of viral proteins across the nuclear envelope. In this study, we show that ivermectin inhibits HAdV-C5 early gene transcription, early and late protein expression, genome replication, and production of infectious viral progeny. Similarly, ivermectin inhibits genome replication of HAdV-B3, a clinically important pathogen responsible for numerous recent outbreaks. Mechanistically, we show that ivermectin disrupts binding of the viral E1A protein to Imp-α without affecting the interaction between Imp-α and Imp-β1. Our results further extend ivermectin’s broad antiviral activity and provide a mechanistic underpinning for its mode of action as an inhibitor of cellular Imp-α/β1-mediated nuclear import.

IMPORTANCE Human adenoviruses (HAdVs) represent a ubiquitous and clinically important pathogen without an effective antiviral treatment. HAdV infections typically cause mild symptoms; however, individuals such as children, those with underlying conditions, and those with compromised immune systems can develop severe disseminated disease. Our results demonstrate that ivermectin, an FDA-approved antiparasitic agent, is effective at inhibiting replication of several HAdV types in vitro. This is in agreement with the growing body of literature suggesting ivermectin has broad antiviral activity. This study expands our mechanistic knowledge of ivermectin by showing that ivermectin targets the ability of importin-α (Imp-α) to recognize nuclear localization sequences, without effecting the Imp-α/β1 interaction. These data also exemplify the applicability of targeting host factors upon which viruses rely as a viable antiviral strategy.

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Inhibition of Human Adenovirus Replication by the Importin α/β1 Nuclear Import Inhibitor Ivermectin
Cason R. King, Tanner M. Tessier, Mackenzie J. Dodge, Jason B. Weinberg, Joe S. Mymryk
Journal of Virology Aug 2020, 94 (18) e00710-20; DOI: 10.1128/JVI.00710-20

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Inhibition of Human Adenovirus Replication by the Importin α/β1 Nuclear Import Inhibitor Ivermectin
Cason R. King, Tanner M. Tessier, Mackenzie J. Dodge, Jason B. Weinberg, Joe S. Mymryk
Journal of Virology Aug 2020, 94 (18) e00710-20; DOI: 10.1128/JVI.00710-20
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KEYWORDS

E1A
adenoviruses
ivermectin
nuclear import

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