Cover photograph: Rotavirus genome replication, packaging of virus genome segments in viral cores, and double-layered particle (DLP) formation take place in membraneless cytosolic inclusions called viroplasms. DLPs bud from viroplasms to the rough endoplasmic reticulum (RER) to become mature triple-layered particles (TLPs). During this process, a transient lipidic membrane surrounds immature TLPs. The image represents high-definition transmission electron microscopy of a viroplasm (blue) at 6 h postinfection surrounded by RER (green) containing TLPs (red). After glutaraldehyde fixation, samples were stained with osmium tetroxide and potassium ferrocyanide. Acquired images were colored using Adobe Photoshop. (See related article in October 2019, vol. 93, no. 19, e01062-19.) (Copyright © 2019 American Society for Microbiology. All Rights Reserved.)