HIV-1 Vif's Capacity To Manipulate the Cell Cycle Is Species Specific

HIV-1 Vif causes CPE and G₂/M cell cycle arrest in HeLa cells but not in 3T3.CX cells. HeLa and 3T3.CX cells were infected with either Vif⁺ or ΔVif R-E-/CFP viruses. (A) Visualization of infected HeLa and 3T3.CX cells. Bars, ∼100 μm. For all CFP images, all CFP fluorescence was pseudocolored black on a white background to maximize contrast. Accordingly, uninfected cells or infected cells expressing CFP at or below background are shown as white. Only HeLa cells infected with a Vif⁺ virus underwent cell rounding (insets), while HeLa cells infected with ΔVif viruses maintained normal cell morphology despite infection. 3T3.CX cells maintained normal cell morphology regardless of which virus (Vif⁺ or ΔVif) was used to infect cells. (B) Both HeLa and 3T3.CX cells were infected with Vif⁺ R-E-/CFP or ΔVif R-E-/CFP as indicated, harvested, and analyzed using flow cytometry to quantify per-cell DNA content using propidium iodide staining. For flow histograms, the leftmost peak indicates DNA content prior to S phase (G₁), with the rightmost peak demonstrating cells with duplicated DNA content (G₂/M). The blue curve between the G₁ and G₂/M peaks represents the S phase. Quantification of G₂/M status for each condition is presented on the right of the flow histograms. Infecting HeLa cells with Vif⁺ viruses induced G₂/M cell cycle arrest, while ΔVif virus did not, consistent with the observed cell morphology changes presented in panel A. 3T3.CX cells were nonresponsive to either virus and did not undergo G₂/M cell cycle arrest. Error bars represent the standard deviations from the means (n = 3). (C) Treating HeLa or 3T3.CX cells with 1 μM nocodazole for 24 h induced potent G₂/M cell cycle arrest and cell rounding changes in both cell lines (rightmost images). Error bars represent the standard deviations from the means (n = 3).

Vif alone is sufficient to induce cell rounding and G₂/M cell cycle arrest in HeLa cells but not in 3T3.CX cells. (A) Depiction of bicistronic retroviral vectors that express both codon-optimized Vif (CO-Vif) and cyan fluorescent protein (CFP) from reading frames separated by an internal ribosomal entry site (IRES). Three independent constructs were studied: CO-Vif_NL4-3 (induces G₂/M arrest), C114S CO-Vif_NL4-3 (catalytically inactive, should not induce G₂/M arrest), and CO-Vif_HXB2 (should not induce G₂/M arrest). (B) Western blot analysis confirming that each construct expressed CO-Vif in addition to CFP, assayed in HeLa cells. (C) Visualization of HeLa cells and 3T3 cells transduced with each construct, separately. Bar, ∼100 μm. HeLa cells that expressed CO-Vif_NL4-3 underwent marked cell rounding (insets), while HeLa cells expressing C114S CO-Vif_NL4-3 and CO-Vif_HXB2 maintained normal cell morphology (compare mock [Vector] images to CO-Vif images). 3T3 cells, however, maintained normal cell morphology under every condition. Treatment with 1 μM nocodazole as a G₂/M control induced cell rounding in both cell types. (D) Cell cycle analysis of HeLa and 3T3 cells using flow cytometry confirmed that 3T3 cells failed to undergo G₂/M cell cycle arrest in the presence of CO-Vif_NL4-3 expression. Error bars represent the standard deviations from the means (n = 3).

Vif is capable of degrading hA3G, CBF-β, and PPP2R5D in both HeLa and 3T3 cells. (A) Model of the Vif-APOBEC3G E3 ubiquitin ligase complex based on that proposed by Letko et al. (95). In this model, Vif (cyan) is bound to elongin-C (orange), CBF-β (gray), and CUL5 (purple), with species-specific CUL5 residues near the Vif-binding interface highlighted in yellow. Elongin-B (red) is shown bound to elongin-C but does not bind to Vif directly. Amino acid sequences identifying mouse versus human orthologues of each factor are as indicated in the table. (B) HeLa and 3T3 cell lines stably expressing YFP-hA3G (yellow) were transduced to express increasing amounts of CO-Vif_NL4-3 as indicated. Images show YFP-hA3G degradation at 48 h postransduction, based on the disappearance of YFP fluorescence detected by microscopy using a 10× objective lens for ∼100 cells/field and measured using FIJI/ImageJ2 software. Bar, ∼100 μm. Insets show bright-field image of cells in order to illustrate the consistent cell density at 48 h. Note that YFP-hA3G is practically undetectable at the highest dose (800 μl) of Vif vector, consistent with efficient degradation. (C) Western blot analysis showing relative Vif, YFP-hA3G, CBFβ, and PPP2R5D levels for an experiment identical to that presented in panel B. HSP90 served as a negative control for Vif effects and also as a loading control. (D) Quantification of YFP-hA3G and PPP2R5D levels relative to HSP90 based on quantitative Western blotting (as shown in panel C) and confirming similar, dose-dependent degradation levels in either cell type. Error bars represent the standard deviations from the means (n = 3).

Vif-induced cell cycle arrest is species specific. (A) Images of cell lines derived from humans (HeLa, HOS, U2OS) or a nonhuman primate (COS7) and infected with either Vif⁺ or ΔVif R-E-/mCherry HIV-1 and visualized using a 10× objective lens at 3 days postinfection. Bar, ∼100 μm. Inset images highlight frequent observations of cell rounding and cytopathic effect. (B) Additional informative nonhuman cell lines derived from rodent (mouse L cells, Rat1, Chinese hamster ovary [CHO]), chiropteran (TB1), and avian (DF1) species and infected with Vif⁺ and ΔVif viruses as indicated. Images were collected as described for panel A and are shown for a later time point, 7 days postinfection, to demonstrate that infection by either virus was tolerated for all cell types relative to the HeLa cell control. Bar, ∼100 μm. Inset images highlight the few remaining HeLa cells in culture at 7 days postinfection with the Vif⁺ virus. (C) Quantification of mCherry mean fluorescence intensity (MFI) for whole-field imaging of the cells presented in panel A, demonstrating the loss of mCherry-expressing human cells at 6 days postinfection. Error bars represent the standard deviations from the means for three independent infections. For both panels C and D, the skull and crossbone symbols indicate high levels of cell death (as opposed to reductions to per-cell mCherry fluorescence). (D) Plot of mCherry MFI, as described for panel C, for whole-field imaging of the cells at 7 days postinfection presented in panel B. (E) Western blot analysis for cell lysates as described for Fig. 1E depicting Gag/Gag-Pol expression and cleavage profiles for HeLa and Cos7 cells infected with the indicated viruses and harvested at 6 days postinfection. Equivalent amounts of total protein were loaded into each well, thus confirming that Cos7 cells can tolerate and maintain infection with a Vif⁺ virus over multiple cell divisions, while infected HeLa cells are gradually lost from the culture. (F) Cell cycle analysis as described for Fig. 3, comparing HeLa and Cos7 cells transduced to express CO-Vif_NL4-3 in the absence of other viral factors. Notably, Cos7 cells were largely resistant to HIV-1 Vif-mediated G₂/M cell cycle arrest. Error bars represent the standard deviations from the means (n = 3).