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Genome Replication and Regulation of Viral Gene Expression

Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses

Nicholas S. Eyre, Stephen M. Johnson, Auda A. Eltahla, Maria Aloi, Amanda L. Aloia, Christopher A. McDevitt, Rowena A. Bull, Michael R. Beard
Michael S. Diamond, Editor
Nicholas S. Eyre
aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
bCentre for Cancer Biology, SA Pathology, Adelaide, Australia
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Stephen M. Johnson
aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
bCentre for Cancer Biology, SA Pathology, Adelaide, Australia
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Auda A. Eltahla
cViral Immunology Systems Program, School of Medical Sciences, The Kirby Institute, UNSW, Sydney, Australia
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Maria Aloi
aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
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Amanda L. Aloia
dCell Screen SA, Flinders Centre for Innovation in Cancer, Flinders University, Bedford Park, Australia
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Christopher A. McDevitt
aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
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Rowena A. Bull
cViral Immunology Systems Program, School of Medical Sciences, The Kirby Institute, UNSW, Sydney, Australia
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Michael R. Beard
aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
bCentre for Cancer Biology, SA Pathology, Adelaide, Australia
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Michael S. Diamond
Washington University School of Medicine
Roles: Editor
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DOI: 10.1128/JVI.01455-17
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Article Information

DOI 
https://doi.org/10.1128/JVI.01455-17
PubMed 
28956770

Published By 
American Society for Microbiology Journals
History 
  • Received August 25, 2017
  • Accepted September 21, 2017
  • Published online November 14, 2017.

Copyright & Usage 
Copyright © 2017 American Society for Microbiology. All Rights Reserved .
Editors and / or Reviewers 
  • Michael S. Diamond, Editor, Washington University School of Medicine

Article Versions

  • Accepted Manuscript version (September 27, 2017).
  • You are viewing the most recent version of this article.

Author Information

  1. Nicholas S. Eyrea,b,
  2. Stephen M. Johnsona,b*,
  3. Auda A. Eltahlac,
  4. Maria Aloia*,
  5. Amanda L. Aloiad,
  6. Christopher A. McDevitta,
  7. Rowena A. Bullc and
  8. Michael R. Bearda,b
  1. aResearch Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
  2. bCentre for Cancer Biology, SA Pathology, Adelaide, Australia
  3. cViral Immunology Systems Program, School of Medical Sciences, The Kirby Institute, UNSW, Sydney, Australia
  4. dCell Screen SA, Flinders Centre for Innovation in Cancer, Flinders University, Bedford Park, Australia
  • Address correspondence to Nicholas S. Eyre, nicholas.eyre{at}adelaide.edu.au, or Michael R. Beard, michael.beard{at}adelaide.edu.au.
  • N.S.E. and S.M.J. contributed equally to this article.

  • Citation Eyre NS, Johnson SM, Eltahla AA, Aloi M, Aloia AL, McDevitt CA, Bull RA, Beard MR. 2017. Genome-wide mutagenesis of dengue virus reveals plasticity of the NS1 protein and enables generation of infectious tagged reporter viruses. J Virol 91:e01455-17. https://doi.org/10.1128/JVI.01455-17 .

  • ↵* Present address: Stephen M. Johnson, The Australian Wine Research Institute, Glen Osmond, South Australia, Australia; Maria Aloi, Department of Microbiology, Monash University, Clayton, Victoria, Australia.

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Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses
Nicholas S. Eyre, Stephen M. Johnson, Auda A. Eltahla, Maria Aloi, Amanda L. Aloia, Christopher A. McDevitt, Rowena A. Bull, Michael R. Beard
Journal of Virology Nov 2017, 91 (23) e01455-17; DOI: 10.1128/JVI.01455-17

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Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses
Nicholas S. Eyre, Stephen M. Johnson, Auda A. Eltahla, Maria Aloi, Amanda L. Aloia, Christopher A. McDevitt, Rowena A. Bull, Michael R. Beard
Journal of Virology Nov 2017, 91 (23) e01455-17; DOI: 10.1128/JVI.01455-17
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KEYWORDS

dengue virus
Genome, Viral
Mutagenesis, Insertional
viral nonstructural proteins
virus replication
dengue virus
NS1
mutagenesis
virus replication
virus assembly
electron microscopy

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