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Genetic Diversity and Evolution | Spotlight

The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Romain Gallet, Frédéric Fabre, Yannis Michalakis, Stéphane Blanc
Anne E. Simon, Editor
Romain Gallet
aINRA, UMR BGPI, INRA-CIRAD-SupAgro, Cirad TA-A54/K, Campus International de Baillarguet, Montpellier, France
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Frédéric Fabre
bUMR 1065 Santé et Agroécologie du Vignoble, INRA, Villenave d'Ornon, France
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Yannis Michalakis
cUMR MIVEGEC 5290, CNRS-IRD-Université de Montpellier, IRD, Montpellier, France
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Stéphane Blanc
aINRA, UMR BGPI, INRA-CIRAD-SupAgro, Cirad TA-A54/K, Campus International de Baillarguet, Montpellier, France
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Anne E. Simon
University of Maryland, College Park
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DOI: 10.1128/JVI.00561-17
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Article Information

DOI 
https://doi.org/10.1128/JVI.00561-17
PubMed 
28566384

Published By 
American Society for Microbiology Journals
History 
  • Received April 4, 2017
  • Accepted May 25, 2017
  • Published online July 27, 2017.

Copyright & Usage 
Copyright © 2017 American Society for Microbiology. All Rights Reserved .
Editors and / or Reviewers 
  • Anne E. Simon, Editor, University of Maryland, College Park

Article Versions

  • Accepted Manuscript version (May 31, 2017).
  • You are viewing the most recent version of this article.

Author Information

  1. Romain Galleta,
  2. Frédéric Fabreb,
  3. Yannis Michalakisc and
  4. Stéphane Blanca
  1. aINRA, UMR BGPI, INRA-CIRAD-SupAgro, Cirad TA-A54/K, Campus International de Baillarguet, Montpellier, France
  2. bUMR 1065 Santé et Agroécologie du Vignoble, INRA, Villenave d'Ornon, France
  3. cUMR MIVEGEC 5290, CNRS-IRD-Université de Montpellier, IRD, Montpellier, France
  • Address correspondence to Romain Gallet, rgallet{at}gmail.com.
  • Citation Gallet R, Fabre F, Michalakis Y, Blanc S. 2017. The number of target molecules of the amplification step limits accuracy and sensitivity in ultradeep-sequencing viral population studies. J Virol 91:e00561-17. https://doi.org/10.1128/JVI.00561-17 .

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The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies
Romain Gallet, Frédéric Fabre, Yannis Michalakis, Stéphane Blanc
Journal of Virology Jul 2017, 91 (16) e00561-17; DOI: 10.1128/JVI.00561-17

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The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies
Romain Gallet, Frédéric Fabre, Yannis Michalakis, Stéphane Blanc
Journal of Virology Jul 2017, 91 (16) e00561-17; DOI: 10.1128/JVI.00561-17
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KEYWORDS

DNA sequencing
FBNSV
faba bean
Medicago truncatula
next-generation sequencing
number of target molecules
rolling-circle amplification
sequencing accuracy
sequencing sensitivity

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