Genetic Diversity and Evolution | Spotlight

The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Romain Gallet, Frédéric Fabre, Yannis Michalakis, Stéphane Blanc
Anne E. Simon, Editor
DOI: 10.1128/JVI.00561-17

Article Information

DOI 
https://doi.org/10.1128/JVI.00561-17
PubMed 
28566384
Published By 
American Society for Microbiology Journals
History 
  • Received April 4, 2017
  • Accepted May 25, 2017
  • Published online July 27, 2017.
Copyright & Usage 
Copyright © 2017 American Society for Microbiology. All Rights Reserved .
Editors and / or Reviewers 
  • Anne E. Simon, Editor, University of Maryland, College Park

Article Versions

Author Information

  1. Romain Galleta,
  2. Frédéric Fabreb,
  3. Yannis Michalakisc and
  4. Stéphane Blanca
  1. aINRA, UMR BGPI, INRA-CIRAD-SupAgro, Cirad TA-A54/K, Campus International de Baillarguet, Montpellier, France
  2. bUMR 1065 Santé et Agroécologie du Vignoble, INRA, Villenave d'Ornon, France
  3. cUMR MIVEGEC 5290, CNRS-IRD-Université de Montpellier, IRD, Montpellier, France
  • Address correspondence to Romain Gallet, rgallet{at}gmail.com.

  • Citation Gallet R, Fabre F, Michalakis Y, Blanc S. 2017. The number of target molecules of the amplification step limits accuracy and sensitivity in ultradeep-sequencing viral population studies. J Virol 91:e00561-17. https://doi.org/10.1128/JVI.00561-17 .

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KEYWORDS

DNA sequencing
FBNSV
faba bean
Medicago truncatula
next-generation sequencing
number of target molecules
rolling-circle amplification
sequencing accuracy
sequencing sensitivity

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