ABSTRACT
Ebolavirus, a deadly hemorrhagic fever virus, was thought to enter cells through endolysosomes harboring its glycoprotein receptor, Niemann-Pick C1. However, an alternate model was recently proposed in which ebolavirus enters through a later NPC1-negative endosome that contains two-pore Ca2+ channel 2 (TPC2), a newly identified ebolavirus entry factor. Here, using live cell imaging, we obtained evidence that in contrast to the new model, ebolavirus enters cells through endolysosomes that contain both NPC1 and TPC2.
FOOTNOTES
- Received 18 July 2015.
- Accepted 6 October 2015.
- Accepted manuscript posted online 14 October 2015.
- Address correspondence to Judith M. White, jw7g{at}virginia.edu.
Citation Simmons JA, D'Souza RS, Ruas M, Galione A, Casanova JE, White JM. 2016. Ebolavirus glycoprotein directs fusion through NPC1+ endolysosomes. J Virol 90:605–610. doi:10.1128/JVI.01828-15.
Supplemental material for this article may be found at http://dx.doi.org/10.1128/JVI.01828-15.
- Copyright © 2015, American Society for Microbiology. All Rights Reserved.
