Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Minireviews
    • JVI Classic Spotlights
    • Archive
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JVI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Virology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Minireviews
    • JVI Classic Spotlights
    • Archive
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JVI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Pathogenesis and Immunity

Domestic Pigs Are Susceptible to Infection with Influenza B Viruses

Zhiguang Ran, Huigang Shen, Yuekun Lang, Elizabeth A. Kolb, Nuri Turan, Laihua Zhu, Jingjiao Ma, Bhupinder Bawa, Qinfang Liu, Haixia Liu, Megan Quast, Gabriel Sexton, Florian Krammer, Ben M. Hause, Jane Christopher-Hennings, Eric A. Nelson, Juergen Richt, Feng Li, Wenjun Ma
R. M. Sandri-Goldin, Editor
Zhiguang Ran
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
cDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Huigang Shen
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuekun Lang
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth A. Kolb
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nuri Turan
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laihua Zhu
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
cDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingjiao Ma
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bhupinder Bawa
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qinfang Liu
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haixia Liu
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Megan Quast
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabriel Sexton
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Florian Krammer
dDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ben M. Hause
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jane Christopher-Hennings
cDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric A. Nelson
cDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juergen Richt
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Feng Li
aDepartment of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA
cDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wenjun Ma
bDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. M. Sandri-Goldin
Roles: Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JVI.00059-15
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • FIG 1
    • Open in new tab
    • Download powerpoint
    FIG 1

    Transmission of B/Brisbane/60/2008 virus from inoculated pigs to contact animals. Eight pigs were inoculated intratracheally and intranasally with influenza B/Brisbane/60/2008 virus at a dose of 3 × 106 TCID50/ml. Four contact pigs were comingled with the challenged pigs at 2 days postinfection (dpi) to investigate viral transmission. Six out of 8 inoculated pigs shed virus by nasal cavity, and 2 inoculated pigs did not shed virus. Nasal shedding was detectable in 1 out of 4 contact pigs. Solid lines represent inoculated pigs, while dotted lines are used to represent contact animals.

  • FIG 2
    • Open in new tab
    • Download powerpoint
    FIG 2

    Microscopic lung sections from pigs infected with IBVs at 5 days postinfection (dpi). (A) Control pigs. The bronchioles are lined by normal cuboidal epithelium (arrow), and the alveoli are clear (star). (B and C) Infected pigs. Alveolitis is observed in pigs infected with either influenza B/Brisbane/60/2008 (B) or B/Yamagata/16/1988 (C) viruses. Multifocal areas of alveolitis are seen and are characterized by the presence of degenerate and intact neutrophils within the alveolar lumen (star), and there is a thickening of alveolar septa caused by inflammation. No bronchiolar epithelial necrosis is observed in any of the infected pigs. Bars, 50 μm.

Tables

  • Figures
  • Additional Files
  • TABLE 1

    Cross-reactivity between influenza A and B viruses tested by HI assay

    AntigenHI titer toa:
    SwH1N1pdmH1N1H3N2vB/YamagataB/VictoriaNegative control
    A/swine/IA/73 (H1N1)160NDND<10<10<10
    A/California/07/2009 (pdmH1N1)ND1,280<10<10<10<10
    A/Minnesota/11/2010 (H3N2v)ND<10640<10<10<10
    B/Yamagata lineage (ether treated)
        B/Wisconsin/1/2010<10<10<101,28040<10
        B/Brisbane/60/2008<10<10<10405,120<10
    • ↵a ND, not determined.

  • TABLE 2

    Summary of the serological survey from 20 virus-positive domestic swine farms by HI assay

    Yr of sampling and farmNo. of sera positivea for B/Yamagata lineagebPositive (%)No. of sera positive for B/Victoria lineagecPositive (%)
    2010-2011
        11-A0/2705/27 (557)18.5
        11-B0/1002/10 (905)20.0
        11-C0/701/7 (640)16.7
        11-D0/3001/30 (1,280)3.3
    2012
        12-A0/1201/12 (40)8.3
        12-B4/12 (67)d33.31/12 (40)8.3
        12-E0/1605/16 (243)31.3
        12-G1/11 (40)9.10/110
        12-L1/13 (40)7.70/130
        12-T2/12 (40)16.70/120
        12-V1/12 (80)8.30/120
        12-W2/10 (40)20.00/100
        S12-A2/10 (40)20.01/10 (160)10.0
        S12-B5/10 (61)50.00/100
        S12-D1/10 (160)10.00/100
        S12-E1/10 (40)10.00/100
        S12-G0/1204/10 (48)40.0
        S12-J0/501/5 (320)20.0
        S12-K4/12 (40)33.30/120
        S12-S1/8 (1,280)12.51/8 (160)12.5
    • ↵a Data are positive sample numbers out of the total number tested.

    • ↵b Yamagata lineage B/Wisconsin/1/2010 was used.

    • ↵c Victoria lineage B/Brisbane/60/2008 was used.

    • ↵d Numbers in parentheses indicate geometric mean HI titers for samples with a value of ≥40.

  • TABLE 3

    Comparison of neutralization and HI antibody titers for a subset of serum samples

    Serum sampleNeutralization titerHI titer
    B/Florida/04/06 (B/Yamagata)B/Brisbane/60/08 (B/Victoria)B/Georgia/07/10 (B/Victoria)B/Georgia/07/10 (B/Victoria)B/Florida/04/06 (B/Yamagata)B/Brisbane/60/08 (B/Victoria)
    Gnotobiotic piglet serum≤5≤5≤5≤5≤5≤5
    S1 (11-A)≤5≥1,280640320≤5320
    S2 (11-A)≤5≤5≤5≤5≤5≤5
    S3 (11-A)≤5≤5≤5≤5≤5≤5
    S4 (11-A)≤5≤5≤5≤5≤5≤5
    S5 (11-A)≤5≥1,280≥1,280320≤5640
    S6 (11-A)≤5≥1,280≥1,280320≤5640
    S7 (11-A)≤5≤5≤5≤5≤5≤10
    S8 (11-A)≤5≥1,280≥1,280320≤5640
    S9 (11-A)≤5≤5≤5≤5≤5≤5
    S10 (11-A)≤5≤5≤5≤5≤5≤5
    S11(11-A)≤5≤5≤5≤5≤5≤5
    S12 (11-A)≤5≤5≤5≤5≤5≤5
    S13 (11-B)≤5≤5≤5≤5≤5≤5
    S14 (11-B)≤5≥1,280640320≤51,280
    S15 (11-B)≤5≥1,280≥1,280320≤5640
    S16 (11-C)≤5≤5≤5≤5≤5≤5
    S17 (11-C)≤5≤5≤5≤5≤5≤5
    S18 (11-C)≤5≤5≤5≤5≤5≤5
    S19 (11-C)≤5≥1,280≥1,280320≤5640
    S20 (11-D)≤5≥1,280≥1,280320≤51,280
  • TABLE 4

    Summary of the serological survey in PRRSV-positive and -negative domestic swine farms by HI assay

    Farm and PRRSV statusNo. of sera positivea for B/Yamagata lineagebPositive (%)No. of sera positive for B/Victoria lineagecPositive (%)
    Positive
        A4/10 (48)d400/100
        B0/900/90
        C0/600/60
        D29/90 (51)32.248/90 (69)53.3
    Negative
        A0/700/70
        B0/900/90
        C0/900/90
        D0/9000/900
    • ↵a Data are the number of positive samples out of the total number tested.

    • ↵b B/Yamagata/16/1988 was used.

    • ↵c B/Brisbane/60/2008 was used.

    • ↵d Numbers in parentheses indicate geometric mean HI titers for samples with a value of ≥40.

  • TABLE 5

    Lung lesions, virus lung replication (BALF virus titer), and seroconversion (HI titers) of infected and contact pigs for the B/Brisbane/60/2008 (Victoria lineage) infection group

    ParameterValue for:
    Infected pigs at:Contact pigs (12 dpc)
    5 dpi14 dpi
    626364656667686970717273
    Lung lesionNoYesYesNoNoYesNoNoNoNoNoNo
    BALF virus titer<12.501.67NDaNDNDNDNDNDNDNDND
    HI titer<10<10<104080160404040<10<10<10
    • ↵a ND, not determined.

  • TABLE 6

    Lung lesions, virus lung replication (BALF virus titer), and seroconversion (HI titers) of infected and contact pigs for the B/Yamagata/16/1988 (Yamagata lineage) infection group

    ParameterValue for:
    Infected pigs at:Contact pigs (12 dpc)
    5 dpi14 dpi
    1234567
    Lung lesionYesYesNoNoNoNoNo
    BALF virus titer<1<1NDaNDNDNDND
    HI titer<10<10804080<10<10
    • ↵a ND, not determined.

Additional Files

  • Figures
  • Tables
  • Supplemental material

    Files in this Data Supplement:

    • Supplemental file 1 -

      Table S1 (Percentages and hemagglutination inhibition titers for serum samples from 52 swine farms.)

      PDF, 319K

PreviousNext
Back to top
Download PDF
Citation Tools
Domestic Pigs Are Susceptible to Infection with Influenza B Viruses
Zhiguang Ran, Huigang Shen, Yuekun Lang, Elizabeth A. Kolb, Nuri Turan, Laihua Zhu, Jingjiao Ma, Bhupinder Bawa, Qinfang Liu, Haixia Liu, Megan Quast, Gabriel Sexton, Florian Krammer, Ben M. Hause, Jane Christopher-Hennings, Eric A. Nelson, Juergen Richt, Feng Li, Wenjun Ma
Journal of Virology Apr 2015, 89 (9) 4818-4826; DOI: 10.1128/JVI.00059-15

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Virology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Domestic Pigs Are Susceptible to Infection with Influenza B Viruses
(Your Name) has forwarded a page to you from Journal of Virology
(Your Name) thought you would be interested in this article in Journal of Virology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Domestic Pigs Are Susceptible to Infection with Influenza B Viruses
Zhiguang Ran, Huigang Shen, Yuekun Lang, Elizabeth A. Kolb, Nuri Turan, Laihua Zhu, Jingjiao Ma, Bhupinder Bawa, Qinfang Liu, Haixia Liu, Megan Quast, Gabriel Sexton, Florian Krammer, Ben M. Hause, Jane Christopher-Hennings, Eric A. Nelson, Juergen Richt, Feng Li, Wenjun Ma
Journal of Virology Apr 2015, 89 (9) 4818-4826; DOI: 10.1128/JVI.00059-15
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About JVI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #Jvirology

@ASMicrobiology

       

 

JVI in collaboration with

American Society for Virology

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0022-538X; Online ISSN: 1098-5514