Article Figures & Data
Figures
- FIG 1
Replication of SIVsmE543-3 and SIVsmE543-3 S37S98 in macaques with the TRIM5TFP/TFP genotype. Viral loads were quantified and are shown as RNA copies in rhesus macaques infected with SIVsmE543-3 (red) or SIVsmE543-3 S37S98 (blue). vRNA, viral RNA.
- FIG 2
Survival curves of macaques infected with SIVsmE543-3 or SIVsmE543-3 S37S98. The cumulative survival rates of macaques infected with SIVsmE543-3 (red) or SIVsmE543-3 S37S98 (blue) are shown as Kaplan-Meier curves and were compared by a log rank test (P = 0.0141).
- FIG 3
Peripheral CD4+ T cell kinetics. (A to C) Peripheral CD4+ T cells (A), central memory CD4+ T cells (CD28+ CD95+) (B), and naïve CD4+ T cells (CD28+ CD95−) (C) were quantified by FACS and are shown as the absolute numbers per microliter of blood. (D to F) The kinetics of total CD4+ T cells (D), central memory CD4+ T cells (E), and naïve CD4+ T cells (F) at the acute phase of infection for groups infected with SIVsmE543-3 (red line) or SIVsmE543-3 S37S98 (blue line) were compared by two-way ANOVA.
- FIG 4
Variance of the SIV capsid sequence at chronic and late stages of infection. The nucleotide sequences encoding the capsid domains of SIV Gag cloned from the plasma of macaques Rh824, Rh825, and Rh831 (SIVsmE543-3 infected) and from the plasma of macaques Rh826, Rh827, Rh832, Rh833, and Rh835 (SIVsmE543-3 S37S98 infected) were aligned to the SIVSmE543-3 capsid sequence. The sequences encoding the capsid N-terminal domains are shown. Codons 37 and 98, which are associated with TRIM5α sensitivity, are highlighted in yellow. w.p.i., weeks postinfection.
Tables
- TABLE 1
Summary of clinical and pathological findings
Inoculated virus Macaque MHC genotypea Time to death (weeks p.i.) Clinical and pathological findings Opportunistic agent SIVsmE543-3 Rh824 A08, B01 105 Seizures, interstitial pneumonia, splenomegaly, PML SV40 Rh825 A02, B01 Surviving N/Ab N/A Rh828 A01, A08, B17 Surviving N/A N/A Rh829 Negative Surviving N/A N/A Rh830 A08 Surviving N/A N/A Rh831 B17 95 Lymphoproliferative disorder (kidney, lymph nodes, spleen), enteritis N/Dc SIVsmE543-3 S37S98 Rh826 B01 11 Significant weight loss, diarrhea, severe enterocolitis Brachyspira Rh827 A02, A08 63 Severe diarrhea, severe enterocolitis Cytomegalovirus Rh832 A01, B01 93 Diarrhea, lymphoproliferative disorder (kidney, lymph nodes, spleen), enteritis N/D Rh833 A08, B01, B08 54 Diarrhea, PML, esophagitis, enterocolitis Candida, SV40 Rh834 B17 Surviving N/A N/A Rh835 Negative 41 Severe diarrhea, enterocolitis, focal pyogranulomatous lymphadenitis N/D - TABLE 2
Summary of amino acid substitutions in progressors
Virus and macaque Time point (weeks p.i.)a Capsid amino acid at position: 37 98 SIVsmE543-3 P R Rh824 40 S G 105* S T Rh825 60 S R/I 106* S T Rh831 48 S R 95* S R/S SIVsmE543-3 S37S98 S S Rh826 11* S S Rh827 36 S S 63* S S Rh832 35 S S 93* S S Rh833 54* S S Rh835 41* S S ↵a *, necropsy time point.
