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Virus-Cell Interactions

Cellular Visualization of Macrophage Pyroptosis and Interleukin-1β Release in a Viral Hemorrhagic Infection in Zebrafish Larvae

Mónica Varela, Alejandro Romero, Sonia Dios, Michiel van der Vaart, Antonio Figueras, Annemarie H. Meijer, Beatriz Novoa
R. W. Doms, Editor
Mónica Varela
aMarine Research Institute, CSIC, Vigo, Spain
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Alejandro Romero
aMarine Research Institute, CSIC, Vigo, Spain
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Sonia Dios
aMarine Research Institute, CSIC, Vigo, Spain
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Michiel van der Vaart
bInstitute of Biology, Leiden University, Leiden, The Netherlands
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Antonio Figueras
aMarine Research Institute, CSIC, Vigo, Spain
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Annemarie H. Meijer
bInstitute of Biology, Leiden University, Leiden, The Netherlands
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Beatriz Novoa
aMarine Research Institute, CSIC, Vigo, Spain
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R. W. Doms
Roles: Editor
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DOI: 10.1128/JVI.02056-14
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ABSTRACT

Hemorrhagic viral diseases are distributed worldwide with important pathogens, such as dengue virus or hantaviruses. The lack of adequate in vivo infection models has limited the research on viral pathogenesis and the current understanding of the underlying infection mechanisms. Although hemorrhages have been associated with the infection of endothelial cells, other cellular types could be the main targets for hemorrhagic viruses. Our objective was to take advantage of the use of zebrafish larvae in the study of viral hemorrhagic diseases, focusing on the interaction between viruses and host cells. Cellular processes, such as transendothelial migration of leukocytes, virus-induced pyroptosis of macrophages. and interleukin-1β (Il-1β) release, could be observed in individual cells, providing a deeper knowledge of the immune mechanisms implicated in the disease. Furthermore, the application of these techniques to other pathogens will improve the current knowledge of host-pathogen interactions and increase the potential for the discovery of new therapeutic targets.

IMPORTANCE Pathogenic mechanisms of hemorrhagic viruses are diverse, and most of the research regarding interactions between viruses and host cells has been performed in cell lines that might not be major targets during natural infections. Thus, viral pathogenesis research has been limited because of the lack of adequate in vivo infection models. The understanding of the relative pathogenic roles of the viral agent and the host response to the infection is crucial. This will be facilitated by the establishment of in vivo infection models using organisms such as zebrafish, which allows the study of the diseases in the context of a complete individual. The use of this animal model with other pathogens could improve the current knowledge on host-pathogen interactions and increase the potential for the discovery of new therapeutic targets against diverse viral diseases.

FOOTNOTES

    • Received 17 July 2014.
    • Accepted 2 August 2014.
    • Accepted manuscript posted online 6 August 2014.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/JVI.02056-14.

  • Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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Cellular Visualization of Macrophage Pyroptosis and Interleukin-1β Release in a Viral Hemorrhagic Infection in Zebrafish Larvae
Mónica Varela, Alejandro Romero, Sonia Dios, Michiel van der Vaart, Antonio Figueras, Annemarie H. Meijer, Beatriz Novoa
Journal of Virology Sep 2014, 88 (20) 12026-12040; DOI: 10.1128/JVI.02056-14

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Cellular Visualization of Macrophage Pyroptosis and Interleukin-1β Release in a Viral Hemorrhagic Infection in Zebrafish Larvae
Mónica Varela, Alejandro Romero, Sonia Dios, Michiel van der Vaart, Antonio Figueras, Annemarie H. Meijer, Beatriz Novoa
Journal of Virology Sep 2014, 88 (20) 12026-12040; DOI: 10.1128/JVI.02056-14
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