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Structure and Assembly

Crystal Structure of Enterovirus 71 RNA-Dependent RNA Polymerase Complexed with Its Protein Primer VPg: Implication for a trans Mechanism of VPg Uridylylation

Cheng Chen, Yaxin Wang, Chao Shan, Yuna Sun, Peng Xu, Honggang Zhou, Cheng Yang, Pei-Yong Shi, Zihe Rao, Bo Zhang, Zhiyong Lou
Cheng Chen
aStructural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science, Tsinghua University, Beijing, China
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Yaxin Wang
bNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
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Chao Shan
cCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
dKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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Yuna Sun
bNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
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Peng Xu
aStructural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science, Tsinghua University, Beijing, China
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Honggang Zhou
eEmerging Infection Disease Program, High-Throughput Molecular Drug Discovery Center, Tianjin Joint Academy of Biomedicine and Technology, Tianjin, China
fCollege of Pharmacy and State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China
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Cheng Yang
eEmerging Infection Disease Program, High-Throughput Molecular Drug Discovery Center, Tianjin Joint Academy of Biomedicine and Technology, Tianjin, China
fCollege of Pharmacy and State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China
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Pei-Yong Shi
gWadsworth Center, New York State Department of Health, Albany, New York, USA
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Zihe Rao
aStructural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science, Tsinghua University, Beijing, China
bNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
fCollege of Pharmacy and State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China
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Bo Zhang
cCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
dKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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Zhiyong Lou
aStructural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science, Tsinghua University, Beijing, China
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DOI: 10.1128/JVI.02733-12
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Article Information

DOI 
https://doi.org/10.1128/JVI.02733-12
PubMed 
23487447

Published By 
American Society for Microbiology Journals
History 
  • Received September 29, 2012
  • Accepted March 3, 2013
  • Published online April 24, 2013.

Copyright & Usage 
Copyright © 2013, American Society for Microbiology. All Rights Reserved.

Article Versions

  • Accepted Manuscript version (March 13, 2013).
  • You are viewing the most recent version of this article.

Author Information

  1. Cheng Chena,
  2. Yaxin Wangb,
  3. Chao Shanc,d,
  4. Yuna Sunb,
  5. Peng Xua,
  6. Honggang Zhoue,f,
  7. Cheng Yange,f,
  8. Pei-Yong Shig,
  9. Zihe Raoa,b,f,
  10. Bo Zhangc,d and
  11. Zhiyong Loua
  1. aStructural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science, Tsinghua University, Beijing, China
  2. bNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
  3. cCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
  4. dKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
  5. eEmerging Infection Disease Program, High-Throughput Molecular Drug Discovery Center, Tianjin Joint Academy of Biomedicine and Technology, Tianjin, China
  6. fCollege of Pharmacy and State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China
  7. gWadsworth Center, New York State Department of Health, Albany, New York, USA
  • Address correspondence to Zhiyong Lou, louzy{at}xtal.tsinghua.edu.cn, or Bo Zhang, zhangbo{at}wh.iov.cn.
  • C.C., Y.W., and C.S. contributed equally to this article.

  • P.-Y.S., Z.R., B.Z., and Z.L. are co-senior authors of this article.

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Crystal Structure of Enterovirus 71 RNA-Dependent RNA Polymerase Complexed with Its Protein Primer VPg: Implication for a trans Mechanism of VPg Uridylylation
Cheng Chen, Yaxin Wang, Chao Shan, Yuna Sun, Peng Xu, Honggang Zhou, Cheng Yang, Pei-Yong Shi, Zihe Rao, Bo Zhang, Zhiyong Lou
Journal of Virology Apr 2013, 87 (10) 5755-5768; DOI: 10.1128/JVI.02733-12

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Crystal Structure of Enterovirus 71 RNA-Dependent RNA Polymerase Complexed with Its Protein Primer VPg: Implication for a trans Mechanism of VPg Uridylylation
Cheng Chen, Yaxin Wang, Chao Shan, Yuna Sun, Peng Xu, Honggang Zhou, Cheng Yang, Pei-Yong Shi, Zihe Rao, Bo Zhang, Zhiyong Lou
Journal of Virology Apr 2013, 87 (10) 5755-5768; DOI: 10.1128/JVI.02733-12
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