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Pathogenesis and Immunity

Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis

Stacy R. Finkbeiner, Yan Li, Susan Ruone, Christina Conrardy, Nicole Gregoricus, Denise Toney, Herbert W. Virgin, Larry J. Anderson, Jan Vinjé, David Wang, Suxiang Tong
Stacy R. Finkbeiner
1Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110
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Yan Li
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Susan Ruone
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Christina Conrardy
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Nicole Gregoricus
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Denise Toney
3Commonwealth of Virginia Division of Consolidated Laboratory Services, Richmond, Virginia 23219
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Herbert W. Virgin
1Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110
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Larry J. Anderson
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Jan Vinjé
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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David Wang
1Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110
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  • For correspondence: davewang@borcim.wustl.edu stong@cdc.gov
Suxiang Tong
2Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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  • For correspondence: davewang@borcim.wustl.edu stong@cdc.gov
DOI: 10.1128/JVI.00998-09
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ABSTRACT

The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. Phylogenetic analysis demonstrated that this virus, named astrovirus VA1 (AstV-VA1), is highly divergent from all previously described astroviruses. Based on RT-PCR, specimens from multiple patients in this outbreak were unequivocally positive for Ast-VA1.

  • Copyright © 2009 American Society for Microbiology
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Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis
Stacy R. Finkbeiner, Yan Li, Susan Ruone, Christina Conrardy, Nicole Gregoricus, Denise Toney, Herbert W. Virgin, Larry J. Anderson, Jan Vinjé, David Wang, Suxiang Tong
Journal of Virology Sep 2009, 83 (20) 10836-10839; DOI: 10.1128/JVI.00998-09

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Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis
Stacy R. Finkbeiner, Yan Li, Susan Ruone, Christina Conrardy, Nicole Gregoricus, Denise Toney, Herbert W. Virgin, Larry J. Anderson, Jan Vinjé, David Wang, Suxiang Tong
Journal of Virology Sep 2009, 83 (20) 10836-10839; DOI: 10.1128/JVI.00998-09
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  • Top
  • Article
    • ABSTRACT
    • Details of outbreak.
    • Genome amplification and sequencing.
    • Genome analysis.
    • Phylogenetic analysis.
    • RT-PCR screening for AstV-VA1.
    • Nucleotide sequence accession number.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

Astroviridae Infections
Child Day Care Centers
Disease Outbreaks
gastroenteritis
Mamastrovirus

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